Smith M D, Wuthiekanun V, Walsh A L, Teerawattanasook N, Desakorn V, Suputtamongkol Y, Pitt T L, White N J
Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
J Clin Pathol. 1995 Feb;48(2):174-6. doi: 10.1136/jcp.48.2.174.
A latex agglutination test for the detection of Pseudomonas pseudomallei antigen in urine was evaluated for the rapid diagnosis of melioidosis. With unconcentrated urine, antigen was detected in only 18% of patients with melioidosis overall. However, when urine was concentrated 100-fold, antigen was detected in 47% overall and in 67% of patients with septicaemia or disseminated infection, in whom a rapid diagnosis is most important. The specificity of the test was 100%. These results compared favourably with an enzyme immunoassay. This latex agglutination test is a simple, rapid and highly specific method of diagnosing melioidosis, and will be particularly useful in areas with limited laboratory facilities.
为快速诊断类鼻疽，对一种用于检测尿液中类鼻疽杆菌抗原的乳胶凝集试验进行了评估。对于未浓缩的尿液，总体上仅18%的类鼻疽患者能检测到抗原。然而，当尿液浓缩100倍时，总体上47%的患者能检测到抗原，而在败血症或播散性感染患者中这一比例为67%，对这些患者而言快速诊断最为重要。该试验的特异性为100%。这些结果与酶免疫测定法相比更具优势。这种乳胶凝集试验是诊断类鼻疽的一种简单、快速且高度特异的方法，在实验室设施有限的地区将特别有用。
