基于一种胞外多糖特异性单克隆抗体，通过乳胶凝集法快速鉴定类鼻疽伯克霍尔德菌。
Rapid identification of Burkholderia pseudomallei by latex agglutination based on an exopolysaccharide-specific monoclonal antibody.
作者信息
Steinmetz I, Reganzerowski A, Brenneke B, Haussler S, Simpson A, White N J
机构信息
Institute of Medical Microbiology, Hannover Medical School, 30625 Hannover, Germany.
出版信息
J Clin Microbiol. 1999 Jan;37(1):225-8. doi: 10.1128/JCM.37.1.225-228.1999.
Abstract
We recently identified a constitutively expressed exopolysaccharide of Burkholderia pseudomallei which is composed of a unique linear tetrasaccharide repeating unit consisting of three galactose residues and one 3-deoxy-D-manno-2-octulosonic acid residue. In this study we developed a latex agglutination test based on monoclonal antibody 3015, which is specific for this exopolysaccharide, and evaluated this test for rapid identification of B. pseudomallei grown on agar plates. All 74 environmental and clinical B. pseudomallei strains tested, originating from different areas of Southeast Asia, northern Australia, and Africa, showed a strong and specific agglutination. B. pseudomallei-like organisms and a variety of other bacteria did not react. In conclusion this monoclonal antibody-based test is a simple, rapid, and highly specific method for identifying B. pseudomallei culture isolates from different geographic areas.
摘要
我们最近鉴定出一种由伯克霍尔德菌属假鼻疽杆菌组成型表达的胞外多糖，其由独特的线性四糖重复单元构成，该单元包含三个半乳糖残基和一个3-脱氧-D-甘露糖-2-辛酮糖酸残基。在本研究中，我们基于对该胞外多糖具有特异性的单克隆抗体3015开发了一种乳胶凝集试验，并评估了该试验用于快速鉴定琼脂平板上生长的假鼻疽杆菌的能力。所有74株经检测的环境和临床假鼻疽杆菌菌株，源自东南亚、澳大利亚北部和非洲的不同地区，均呈现出强烈且特异性的凝集反应。类假鼻疽杆菌及多种其他细菌无反应。总之，这种基于单克隆抗体的试验是一种用于从不同地理区域鉴定假鼻疽杆菌培养分离株的简单、快速且高度特异的方法。
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