Algermissen B, Bauer F, Schadendorf D, Kropp J D, Czarnetzki B M
Department of Dermatology, University Clinics Rudolf Virchow, FU Berlin, Germany.
Exp Dermatol. 1994 Dec;3(6):290-7. doi: 10.1111/j.1600-0625.1994.tb00291.x.
In order to gain insights into the dynamics of mast cell subpopulations in normal and diseased skin, a novel enzyme-histochemical double and triple staining method was employed that allowed the detection of metachromasia (toluidine blue) and the mast cell proteases tryptase and chymase within the same cell. Cryostat sections were used of skin biopsies from the following specimens: normal skin (N = 4), psoriasis (N = 13), atopic eczema (N = 7), lichen planus (N = 6), interferon alpha 2a injection sites (N = 1) of a leukemic infiltrate and corresponding normal skin of the same patient before and after treatment. (i) Equal numbers of tryptase- and chymase-positive mast cells (MCTC) were obtained in all normal and diseased specimens in papillary and reticular dermis, with threefold increases around appendages. (ii) Tryptase-positive mast cells (MCT) were absent in normal skin, but were markedly increased in a disease-specific pattern within the papillary dermis, the inflammatory infiltrate and around appendages. (iii) Marked increases of MCT were also noted at interferon injection sites within the leukemic infiltrate, but not in the normal skin of the same patient. These data suggest that disease-dependent mast cell dynamics involve only MCT in cutaneous inflammation and that MCT numbers are controlled by distinct, disease-specific local tissue factors.
为深入了解正常皮肤和患病皮肤中肥大细胞亚群的动态变化，采用了一种新型酶组织化学双重和三重染色方法，该方法能够在同一细胞内检测异染性（甲苯胺蓝）以及肥大细胞蛋白酶类胰蛋白酶和糜蛋白酶。使用了以下标本皮肤活检的冰冻切片：正常皮肤（N = 4）、银屑病（N = 13）、特应性皮炎（N = 7）、扁平苔藓（N = 6）、白血病浸润的干扰素α2a注射部位（N = 1）以及同一患者治疗前后相应的正常皮肤。（i）在所有正常和患病标本的乳头层和网状真皮中，获得了数量相等的类胰蛋白酶和类糜蛋白酶阳性肥大细胞（MCTC），在附属器周围增加了三倍。（ii）正常皮肤中不存在类胰蛋白酶阳性肥大细胞（MCT），但在乳头层真皮、炎症浸润部位及附属器周围以疾病特异性模式显著增加。（iii）在白血病浸润部位的干扰素注射部位也观察到MCT显著增加，但在同一患者的正常皮肤中未增加。这些数据表明，疾病依赖性肥大细胞动态变化在皮肤炎症中仅涉及MCT，且MCT数量受不同的、疾病特异性的局部组织因子控制。
