Naukkarinen A, Harvima I T, Aalto M L, Harvima R J, Horsmanheimo M
Department of Pathology, University of Kuopio, Finland.
Arch Dermatol Res. 1991;283(7):433-7. doi: 10.1007/BF00371778.
The aim of the present study was to test further our previous hypothesis that the inflammatory reaction in psoriasis is neurogenic. For this purpose, contact sites between mast cells and sensory nerves were morphometrically analysed in the basement membrane zone, papillary dermis and three dermal zones of lesional/non-lesional psoriatic and lichen planus skin as well as in healthy control skin. The analyses were made on sections stained with a histochemical double stain developed for this study. With the double stain, active mast cell tryptase was stained blue enzyme histochemically, and the sensory nerves black using specific monoclonal anti-neurofilament antibodies with immunogold. In psoriatic lesions, both mast cells and mast cell--nerve contacts were markedly more frequent in the basement membrane zone and in the papillary dermis when compared with the corresponding areas in the other groups. Mast cell numbers were increased in both lesional and symptom-free skin in lichen planus, but no increase was found in the mast cell--nerve contacts. Increased contacts between mast cells and sensory nerves indicate that the elements exist for neurogenic inflammation in psoriatic lesions. These increased contacts are not due to the extensive inflammatory reaction only, because they were not observed in lichen planus lesions.
本研究的目的是进一步验证我们之前的假设，即银屑病中的炎症反应是神经源性的。为此，我们对肥大细胞与感觉神经之间的接触部位进行了形态计量学分析，分析对象包括病变/非病变银屑病皮肤、扁平苔藓皮肤以及健康对照皮肤的基底膜带、乳头真皮层和三个真皮层。分析是在为本研究开发的组织化学双重染色切片上进行的。使用这种双重染色，活性肥大细胞类胰蛋白酶通过酶组织化学染成蓝色，感觉神经则使用特异性抗神经丝单克隆抗体和免疫金染成黑色。与其他组的相应区域相比，在银屑病病变中，基底膜带和乳头真皮层中的肥大细胞以及肥大细胞与神经的接触明显更为频繁。扁平苔藓的病变皮肤和无症状皮肤中的肥大细胞数量均增加，但肥大细胞与神经的接触未见增加。肥大细胞与感觉神经之间接触的增加表明，银屑病病变中存在神经源性炎症的要素。这些增加的接触并非仅由广泛的炎症反应所致，因为在扁平苔藓病变中未观察到这种情况。
