抗大肠杆菌O6孔蛋白抗体与泌尿病原体发生交叉反应。

Antibodies to Escherichia coli 06 porins cross-react with urinary pathogens.

作者信息

Kawahara M, Human L G, Winningham J S, Domingue G J

机构信息

Department of Urology, Tulane University School of Medicine, New Orleans, Louisiana, USA.

出版信息

Immunobiology. 1994 Dec;192(1-2):65-76. doi: 10.1016/S0171-2985(11)80408-0.

DOI:10.1016/S0171-2985(11)80408-0

PMID:7538488

Abstract

Antibodies to partially purified E. coli 06 35-40 KDa porin trimers recognized the reactive epitopes in the intact porin surface molecule present in various wild-type, heterologous, urinary pathogens. The presence of lipopolysaccharide in the membrane did not shield the antibody binding sites. The reactivity was shown to be specific for porins since LPS-absorbed porin antisera reacted with porins on immunoblots and showed no reactivity with LPS. Additionally, the cross-reactions were abolished by absorption of the porin antisera with E. coli 06 containing porin trimers. These data strengthen the rationale for exploring the enhancement of immunoprotection by monoclonal antibodies to specific immunoreactive antigens in the porin molecule.

摘要

针对部分纯化的大肠杆菌06 35 - 40千道尔顿孔蛋白三聚体的抗体，能够识别存在于各种野生型、异源尿路病原体完整孔蛋白表面分子中的反应性表位。膜中脂多糖的存在并未掩盖抗体结合位点。已证明这种反应性对孔蛋白具有特异性，因为经脂多糖吸收的孔蛋白抗血清在免疫印迹中与孔蛋白发生反应，而与脂多糖无反应。此外，通过用含孔蛋白三聚体的大肠杆菌06吸收孔蛋白抗血清，交叉反应被消除。这些数据强化了探索通过针对孔蛋白分子中特定免疫反应性抗原的单克隆抗体来增强免疫保护作用的理论依据。