Dun N J, Dun S L, Chiba T, Förstermann U
Department of Anatomy and Neurobiology, Medical College of Ohio, Toledo 43614, USA.
Neuroscience. 1995 Mar;65(1):231-9. doi: 10.1016/0306-4522(94)00455-e.
Chronic (5-14 days) preganglionic denervation of the rat superior cervical ganglia by sectioning the cervical sympathetic trunk resulted in a time-related partial or complete loss of nitric oxide synthase (isoform I)-immunoreactive fibers and terminals surrounding many sympathetic ganglionic neurons. Unexpectedly, denervation unmasked many varicose nitric oxide synthase-immunoreactive fibers, some of which could be traced the entire length of the superior cervical ganglia. Injection of the retrograde tracer Fluorogold into the superior cervical ganglia labeled a population of nodose ganglion cells and of dorsal root ganglion cells from C8 to T3 segments. When the same sections were processed for nitric oxide synthase-immunoreactivity, 40% of the Fluorogold-containing nodose ganglion cells also expressed nitric oxide synthase-immunoreactivity, whereas colocalization was observed in only a few dorsal root ganglion cells. Similarly, injection of Fluorogold into denervated superior cervical ganglia labeled a population of nodose ganglion cells. Sectioning of all nerve trunks associated with the superior cervical ganglion prior to injection of Fluorogold, except the cervical sympathetic trunk, resulted in no detectable labeling of Fluorogold in the ipsilateral nodose ganglion cells. These results indicate that a population of rat nodose ganglion cells contain nitric oxide synthase and that some of these neurons project their axons through the superior cervical ganglion and terminate in the peripheral target tissues. The possibility that nitric oxide synthase-immunoreactive vagal afferent fibers may participate in nociception is considered.
通过切断颈交感干对大鼠颈上神经节进行慢性（5 - 14天）节前去神经支配，导致围绕许多交感神经节神经元的一氧化氮合酶（同工型I）免疫反应性纤维和终末出现与时间相关的部分或完全丧失。出乎意料的是，去神经支配揭示出许多曲张的一氧化氮合酶免疫反应性纤维，其中一些纤维可沿颈上神经节的全长追踪。将逆行示踪剂荧光金注入颈上神经节，标记了一群结状神经节细胞以及来自C8至T3节段的背根神经节细胞。当对相同切片进行一氧化氮合酶免疫反应性处理时，40%含有荧光金的结状神经节细胞也表达一氧化氮合酶免疫反应性，而在仅少数背根神经节细胞中观察到共定位。同样，将荧光金注入去神经支配的颈上神经节标记了一群结状神经节细胞。在注入荧光金之前，切断与颈上神经节相关的所有神经干（颈交感干除外），导致同侧结状神经节细胞中未检测到荧光金标记。这些结果表明，一群大鼠结状神经节细胞含有一氧化氮合酶，并且这些神经元中的一些将其轴突投射通过颈上神经节并终止于外周靶组织。研究了一氧化氮合酶免疫反应性迷走传入纤维可能参与伤害感受的可能性。
