组胺与豚鼠胰腺中的环磷酸腺苷途径

Histamine and the cAMP pathway in the guinea-pig pancreas.

作者信息

Lajas A I, Pariente J A, Salido G M

机构信息

Department of Physiology, Faculty of Veterinary Science, University of Extremadura, Cáceres, Spain.

出版信息

Cell Signal. 1995 Jan;7(1):57-60. doi: 10.1016/0898-6568(94)00058-j.

DOI:10.1016/0898-6568(94)00058-j

PMID:7538775

Abstract

This study investigates the interaction between histamine and the adenylate cyclase systems involved in the secretion of amylase from the guinea-pig pancreatic lobules. Histamine increased amylase release, reaching a maximum response at 10(-5) M. Similarly, vasoactive intestinal peptide (VIP) evoked significant increase in amylase release, though not in a dose-dependent fashion. When the pancreatic lobules were incubated with histamine in combination with VIP, forskolin or 3-isobutyl-1-methylxanthine (IBMX), amylase secretion was increased as compared to histamine alone. The stimulatory effect of VIP was also increased by the presence of forskolin or IBMX. These findings suggest that in guinea-pig pancreatic lobules, VIP, forskolin and IBMX, agents involved in the cyclic adenosine monophosphate (cAMP) pathway, potentiate histamine stimulated amylase release.

摘要

本研究调查了组胺与参与豚鼠胰腺小叶淀粉酶分泌的腺苷酸环化酶系统之间的相互作用。组胺增加了淀粉酶的释放，在10(-5)M时达到最大反应。同样，血管活性肠肽（VIP）引起淀粉酶释放显著增加，尽管不是呈剂量依赖性。当胰腺小叶与组胺联合VIP、福斯可林或3-异丁基-1-甲基黄嘌呤（IBMX）一起孵育时，与单独使用组胺相比，淀粉酶分泌增加。福斯可林或IBMX的存在也增强了VIP的刺激作用。这些发现表明，在豚鼠胰腺小叶中，参与环磷酸腺苷（cAMP）途径的VIP、福斯可林和IBMX增强了组胺刺激的淀粉酶释放。