Langland J O, Pettiford S M, Jacobs B L
Department of Microbiology, Arizona State University, Tempe 85287-2701, USA.
Protein Expr Purif. 1995 Feb;6(1):25-32. doi: 10.1006/prep.1995.1004.
An improved method for the preparation of a double-stranded RNA-coupled resin is described. Periodate-oxidized double-stranded RNA, in the form of either purified reovirus double-stranded RNA or poly(rl). poly(rC), was covalently attached to agarose-adipic acid hydrazide. The resulting affinity resin had increased specificity for known double-stranded RNA binding proteins, as compared to a similar commercially available resin. The application of this resin in the purification and characterization of double-stranded RNA binding proteins is described.
本文描述了一种制备双链RNA偶联树脂的改进方法。以纯化的呼肠孤病毒双链RNA或聚（rI）·聚（rC）形式存在的高碘酸盐氧化双链RNA共价连接到琼脂糖-己二酸酰肼上。与类似的市售树脂相比，所得亲和树脂对已知双链RNA结合蛋白的特异性有所提高。本文还描述了该树脂在双链RNA结合蛋白纯化和表征中的应用。
