Yamashita H, Ohira Y, Wakatsuki T, Yamamoto M, Kizaki T, Oh-ishi S, Sato Y, Ohno H
Department of Hygiene, National Defense Medical College, Tokorozawa, Japan.
J Appl Physiol (1985). 1995 Feb;78(2):384-7. doi: 10.1152/jappl.1995.78.2.384.
To study the responses of thermogenic activity in brown adipose tissue (BAT) to unloading, male Wistar rats were hindlimb suspended for 10 days. Compared with control rats, a significant increase in the BAT-to-body mass ratio and considerable differences in chemical components in BAT were observed in the hindlimb-suspended rats. These findings indicate a marked increase in the thermogenic capacity in BAT of the experimental group. Likewise, the thermogenic activity (which was assessed by guanosine 5'-diphosphate binding to BAT mitochondria) was markedly greater in the mitochondria recovered from BAT of the hindlimb-suspended rats than in those from the control rats (1,610 +/- 450 vs. 202 +/- 132 pmol recovered). Moreover, the uncoupling protein content in the BAT mitochondrial fraction of the hindlimb-suspended rats was significantly higher (1.6-fold) than that in the control rats. As was expected, the uncoupling protein mRNA expression was greater in hindlimb-suspended rats than in control animals. These results suggest that chronic hindlimb suspension leads to an increase in both the thermogenic capacity and the activity in BAT of rats.
为研究棕色脂肪组织（BAT）产热活性对失重的反应，将雄性Wistar大鼠后肢悬吊10天。与对照大鼠相比，后肢悬吊大鼠的BAT与体重比显著增加，且BAT中的化学成分存在显著差异。这些发现表明实验组BAT的产热能力显著增加。同样，从后肢悬吊大鼠的BAT中回收的线粒体的产热活性（通过鸟苷5'-二磷酸与BAT线粒体结合来评估）明显高于对照大鼠（回收量分别为1,610±450与202±132 pmol）。此外，后肢悬吊大鼠的BAT线粒体部分中的解偶联蛋白含量显著高于对照大鼠（1.6倍）。正如预期的那样，后肢悬吊大鼠的解偶联蛋白mRNA表达高于对照动物。这些结果表明，慢性后肢悬吊导致大鼠BAT的产热能力和活性均增加。
