Peek R, van Venrooij W J, Simons F, Pruijn G
Department of Biochemistry, University of Nijmegen, The Netherlands.
Clin Exp Rheumatol. 1994 Nov-Dec;12 Suppl 11:S15-8.
The subcellular distribution of Ro ribonucleoprotein particles (Ro RNPs) and their constituents was determined using mammalian tissue culture cells and Xenopus laevis oocytes. Cell fractionation revealed that Ro RNPs are restricted to the cytoplasm, while the Ro RNP-specific proteins were also present in the nucleus. Transport studies indicated that the protein components of Ro RNPs are actively imported into the nucleus while the RNA component accumulates in the cytoplasm. These results suggest a model for the assembly of Ro RNPs.
利用哺乳动物组织培养细胞和非洲爪蟾卵母细胞，确定了Ro核糖核蛋白颗粒（Ro RNPs）及其成分的亚细胞分布。细胞分级分离显示，Ro RNPs局限于细胞质，而Ro RNP特异性蛋白也存在于细胞核中。转运研究表明，Ro RNPs的蛋白质成分被主动转运到细胞核中，而RNA成分则在细胞质中积累。这些结果提示了一种Ro RNPs组装的模型。
