Smith M R, Biggar S, Hussain M
Department of Medical Oncology, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA.
Cancer Res. 1995 Jun 15;55(12):2640-4.
Prostate specific antigen (PSA) is generally believed to be expressed only by prostate epithelium. If this were true of PSA, RNA, then detecting PSA RNA in cells outside of the prostate would indicate metastasis. PCR can detect rare prostate cancer cells. To enhance sensitivity, we developed "nested primer" PCR to detect PSA RNA. With this method, PSA RNA is present in several non-prostate cell lines, including BG-1 (ovarian), SK-MES-1 (lung), and HL-60 (myeloid leukemia), and some normal blood. A low level of PSA RNA detectable by nested primer PCR is present in some cells of non-prostate origin and may interfere with sensitive methods to detect micrometastases. Transcripts of other genes thought to be organ specific may have similar limitations.
前列腺特异性抗原（PSA）通常被认为仅由前列腺上皮细胞表达。如果PSA RNA也是如此，那么在前列腺以外的细胞中检测到PSA RNA将表明发生了转移。聚合酶链反应（PCR）可以检测到罕见的前列腺癌细胞。为了提高灵敏度，我们开发了“巢式引物”PCR来检测PSA RNA。用这种方法，PSA RNA存在于几种非前列腺细胞系中，包括BG-1（卵巢）、SK-MES-1（肺）和HL-60（髓系白血病），以及一些正常血液中。巢式引物PCR可检测到的低水平PSA RNA存在于一些非前列腺来源的细胞中，可能会干扰检测微转移的灵敏方法。其他被认为是器官特异性的基因转录本可能也有类似的局限性。
