Abrams M E, Balish M J, Brandt C R
Department of Ophthalmology and Visual Sciences, University of Wisconsin Medical School, Madison 53706-1532, USA.
Exp Eye Res. 1995 Feb;60(2):137-42. doi: 10.1016/s0014-4835(95)80003-4.
Treatment of certain human cells with Interferon-alpha (IFN-alpha) induces the synthesis of a 76 kDa protein designated MxA that is involved in resistance to viral infection. We have used a specific cDNA clone and monoclonal Ab to show that MxA is induced in IFN-alpha treated human corneal fibroblast cultures. Mx RNA was increased 23-fold and 45-fold after 5 and 9 h of IFN-alpha treatment, respectively. The MxA protein was detectable by immunoblotting at 5 hr after IFN treatment and peaked at 17 hr. Concentrations of IFN-alpha as low as 1 U/ml induced detectable amounts of MxA, and expression was maximal at 1 x 10(3) U ml-1. These results confirm that MxA synthesis is induced in human corneal fibroblasts treated with IFN-alpha.
用α-干扰素（IFN-α）处理某些人类细胞会诱导合成一种名为MxA的76 kDa蛋白，该蛋白参与抗病毒感染。我们使用了一个特异性cDNA克隆和单克隆抗体来表明，在经IFN-α处理的人角膜成纤维细胞培养物中会诱导产生MxA。IFN-α处理5小时和9小时后，Mx RNA分别增加了23倍和45倍。在IFN处理后5小时通过免疫印迹可检测到MxA蛋白，并在17小时达到峰值。低至1 U/ml的IFN-α浓度即可诱导产生可检测量的MxA，在1×10³ U/ml时表达量最大。这些结果证实，在用IFN-α处理的人角膜成纤维细胞中会诱导MxA的合成。
