Sakai H, Furuta R A, Tokunaga K, Kawamura M, Hatanaka M, Adachi A
Institute for Virus Research, Kyoto University, Japan.
FEBS Lett. 1995 May 29;365(2-3):141-5. doi: 10.1016/0014-5793(95)00444-e.
Structural gene expression of human immunodeficiency virus type 1 (HIV-1) requires a viral regulatory protein, Rev transactivator. We investigated Rev-dependency of HIV-1 gene expression by various reporter systems. Expression of unspliced and single-spliced viral mRNAs was demonstrated to be differentially dependent on the Rev function. This difference of Rev-dependency was found not to be determined by cis-elements in gag, pol, and env coding sequences reported so far, and was lost when the reporter constructs containing minimum elements for Rev-responsiveness such as splice signals and rev responsive element were used for experiments. These findings indicated that the fundamental structure of HIV-1 mRNA was critical for the differential regulation of gene expression by Rev transactivator.
1型人类免疫缺陷病毒（HIV-1）的结构基因表达需要一种病毒调节蛋白Rev反式激活因子。我们通过各种报告系统研究了HIV-1基因表达对Rev的依赖性。结果表明，未剪接和单剪接病毒mRNA的表达对Rev功能的依赖性存在差异。这种Rev依赖性的差异并非由目前报道的gag、pol和env编码序列中的顺式元件所决定，并且当使用含有Rev反应性最小元件（如剪接信号和Rev反应元件）的报告构建体进行实验时，这种差异消失了。这些发现表明，HIV- mRNA的基本结构对于Rev反式激活因子对基因表达的差异调节至关重要。
