Crespi F, England T, Ratti E, Trist D G
Glaxo Research Laboratories, Department of Pharmacology, Verona, Italy.
Neurosci Lett. 1995 Mar 16;188(1):33-6. doi: 10.1016/0304-3940(95)11388-d.
Differential pulse voltammetry and more recently cyclic voltammetry have been successfully used to monitor basal levels of endogenous chemicals by means of treated carbon fibre microbiosensors inserted in specific brain regions. In this study, feasibility of concomitant in vivo recordings of stable electrophysiological signals and basal ascorbate, catecholaminergic and indolaminergic voltammetric peaks at the same cerebral site by means of a single electrically treated carbon fibre micro electrode (microbiosensor) is presented. The results indicate that these two independent techniques can be combined in vivo at a single electrode, and that voltammetric measurements of unstimulated levels of extracellular compounds do not alter concomitant basal cell firing for a period long enough (more than 6 h) to allow pharmacological manipulations.
差分脉冲伏安法以及最近的循环伏安法已成功用于通过插入特定脑区的经处理碳纤维微生物传感器监测内源性化学物质的基础水平。在本研究中，展示了利用单个经电处理的碳纤维微电极（微生物传感器）在同一脑区同时进行稳定电生理信号以及基础抗坏血酸、儿茶酚胺能和吲哚胺能伏安峰的体内记录的可行性。结果表明，这两种独立技术可在体内于单个电极上结合使用，并且细胞外化合物未受刺激水平的伏安测量在足够长的时间段（超过6小时）内不会改变伴随的基础细胞放电，从而允许进行药理学操作。
