Mountford P S, Smith A G
Centre for Animal Biotechnology, School of Veterinary Science, University of Melbourne, Parkville, Victoria, Australia.
Trends Genet. 1995 May;11(5):179-84. doi: 10.1016/S0168-9525(00)89040-X.
Modification of the genetic content of cultured cells or of whole animals is now a key strategy in both basic biological research and applied biotechnology. Yet obtaining the desired level and specificity of expression of an introduced gene remains highly problematic. One solution could be to couple expression of a transgene to that of an appropriate intact genomic locus. The identification and functional characterization of RNA sequences known as internal ribosome entry sites now offer the possibility of achieving precise control of transgene expression through the generation of dicistronic fusion mRNAs.
对培养细胞或整个动物的遗传内容进行修饰，如今已成为基础生物学研究和应用生物技术中的一项关键策略。然而，要获得导入基因所需的表达水平和特异性，仍然存在很大问题。一种解决方案可能是将转基因的表达与合适的完整基因组位点的表达相结合。现在，对被称为内部核糖体进入位点的RNA序列的鉴定和功能表征，为通过产生双顺反子融合mRNA来实现对转基因表达的精确控制提供了可能性。
