Fléjou J F, Muzeau F, Potet F, Lepelletier F, Fékété F, Hénin D
Department of Pathology, Hôpital Beaujon, Clichy, France.
Pathol Res Pract. 1994 Dec;190(12):1141-8. doi: 10.1016/S0344-0338(11)80440-1.
p53 protein has been reported as frequently overexpressed in esophageal and gastric carcinomas. However, the correlation between p53 protein expression and clinico-pathological features of the tumors is debated in this heterogeneous group of cancers. The aim of this study was to establish the prevalence of p53 protein overexpression in a series of resected esophageal squamous carcinomas (n = 78), adenocarcinomas developed on Barrett's esophagus (n = 20), adenocarcinomas of the cardia (n = 36), and adenocarcinomas of the antrum (n = 30), and to correlate this expression with the clinico-pathological and flow-cytometric characteristics of the tumors.
Immunohistochemical staining was performed on frozen sections with a monoclonal antibody directed against wild type and mutated p53 protein (Pab 1801). An adjacent frozen specimen was used for flow cytometric determination of the DNA-ploidy and S phase fraction.
p53 protein nuclear expression was detected in 76% of esophageal squamous carcinomas, in 75% of adenocarcinomas developed in Barretts esophagus, in 56% of adenocarcinomas of the cardia, and in 27% of adenocarcinomas of the antrum. Only the number of positive adenocarcinomas of the antrum was significantly lower when compared to the other three types of tumors (p = 0.001). No significant correlation was observed between p53 protein expression and most of the clinico-pathological and flow-cytometric parameters (sex, age, tobacco smoking, chronic alcohol consumption, size of the tumor, grade of differentiation, depth of infiltration, presence of lymph node metastases, UICC stage, DNA-ploidy, S phase fraction). p53 protein expression was more frequent in Lauren's intestinal adenocarcinomas (67%) when compared to the diffuse type tumors (24%) (p = 0.002).
Our results confirm that overexpression of p53 protein is a common feature of esophageal and gastric carcinomas. The high prevalence of p53 protein overexpression found in cardiac adenocarcinoma when compared to antral adenocarcinoma reinforces the hypothesis of distinct carcinogenetic mechanisms in these two cancers. In particular the lack of correlation between p53 expression and tumor stage suggests that p53 protein overexpression is an early event in these tumors.
据报道，p53蛋白在食管癌和胃癌中经常过度表达。然而，在这一异质性癌症群体中，p53蛋白表达与肿瘤临床病理特征之间的相关性存在争议。本研究的目的是确定一系列切除的食管鳞状细胞癌（n = 78）、巴雷特食管发生的腺癌（n = 20）、贲门腺癌（n = 36）和胃窦腺癌（n = 30）中p53蛋白过度表达的发生率，并将这种表达与肿瘤的临床病理和流式细胞术特征相关联。
用针对野生型和突变型p53蛋白的单克隆抗体（Pab 1801）对冰冻切片进行免疫组织化学染色。相邻的冰冻标本用于流式细胞术测定DNA倍体和S期分数。
在76%的食管鳞状细胞癌、75%的巴雷特食管发生的腺癌、56%的贲门腺癌和27%的胃窦腺癌中检测到p53蛋白核表达。与其他三种类型的肿瘤相比，只有胃窦腺癌的阳性数量显著较低（p = 0.001）。未观察到p53蛋白表达与大多数临床病理和流式细胞术参数（性别、年龄、吸烟、长期饮酒、肿瘤大小、分化程度、浸润深度、淋巴结转移情况、UICC分期、DNA倍体、S期分数）之间存在显著相关性。与弥漫型肿瘤（24%）相比，p53蛋白表达在劳伦氏肠型腺癌（67%）中更常见（p = 0.002）。
我们的结果证实，p53蛋白过度表达是食管癌和胃癌的共同特征。与胃窦腺癌相比，贲门腺癌中p53蛋白过度表达的高发生率强化了这两种癌症存在不同致癌机制的假说。特别是p53表达与肿瘤分期之间缺乏相关性，提示p53蛋白过度表达是这些肿瘤中的早期事件。
