Okamoto Y, Mizuno K, Arakawa K, Kurita A, Nakamura H, Takeuchi K, Yoshioka M
First Department of Internal Medicine, National Defense Medical College, Saitama, Japan.
Am J Card Imaging. 1995 Apr;9(2):57-61.
We attempted to detect the lesion of the damaged endothelium on the atherosclerotic plaque in vivo with dye and angioscope. The plaques were induced on the aortae of rabbits by the continuous mechanical injury with the insertion of the polyethylene tube and feeding with 2% cholesterol-added diet. We observed the some plaques could be stained with 5 x 10(-2) mol/L methylene blue by angioscope. Control aorta, without any stimulations on the aorta, could not be stained. One month after injury and cholesterol feeding the plaques were stained. In such plaques, the lack of the endothelium and the existence of the lipid-containing cell in the intima was observed. However, the plaques of the aorta 6 months after finishing these procedures could not be stained. In these plaques, the morphological characteristics known as the regression of the atheromatous plaque, ie, the regeneration of the endothelium, the thick collagen layer on the plaque and the massive calcification of the plaque were observed. The results obtained were as follows. (1) The atheromatous lesion with the damaged endothelium could be detected in vivo by our vital staining method. (2) The atheromatous plaques stained in vivo are the lesion with complication and enhanced permeability. (3) The slightly stained plaques indicate the lesions with regression and lost high permeability. (4) Our method would be able to add important information on the atheroscrelotic lesions in the clinical situations than that now we perform.
我们试图通过染料和血管镜在体内检测动脉粥样硬化斑块上受损内皮的病变。通过插入聚乙烯管进行连续机械损伤并喂食添加2%胆固醇的饮食，在兔主动脉上诱导形成斑块。我们观察到一些斑块在血管镜下可被5×10(-2)mol/L亚甲蓝染色。未经任何主动脉刺激的对照主动脉不能被染色。损伤和喂食胆固醇1个月后，斑块被染色。在这类斑块中，观察到内膜内皮缺失且存在含脂细胞。然而，完成这些操作6个月后的主动脉斑块不能被染色。在这些斑块中，观察到动脉粥样硬化斑块消退的形态学特征，即内皮再生、斑块上增厚的胶原层和斑块的大量钙化。获得的结果如下。(1)通过我们的活体染色方法可在体内检测到内皮受损的动脉粥样硬化病变。(2)体内被染色的动脉粥样硬化斑块是具有并发症和通透性增强的病变。(3)染色轻微的斑块表明是具有消退且失去高通透性的病变。(4)我们的方法将能够比我们目前在临床情况下所做的为动脉粥样硬化病变增添重要信息。
