Huang C C, Chen K, Huang T Y
Department of Otolaryngology and Head and Neck Surgery, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA.
Eur Arch Otorhinolaryngol. 1995;252(2):86-91. doi: 10.1007/BF00168026.
The olfactory sensory neurons undergo continuous turnover under normal physiological conditions. Injured olfactory sensory neurons are also replaceable. In this study, we investigated cellular differentiation and growth of sensory neurons in the rat's olfactory epithelium after nerve transection by using immunohistochemical staining with polyclonal or monoclonal anti-olfactory marker protein (OMP), anti-c-jun protein and anti-p53 protein antibodies. OMP is found exclusively in olfactory sensory neurons, while c-jun functions as a transcription factor. p53 protein functions as a negative regulator of cellular proliferation related to the apoptotic pathway induced by DNA damage. The olfactory epithelium sections incubated with anti-OMP antibody showed staining of mature neurons and axons in the epithelium. Nerve transection resulted in a significant reduction in neurons labelled with OMP. On the 9th day after operation, our study indicated some recovery with an increasing number of neurons expressing OMP. In control animals without nerve lesions, c-jun protein immunoreactive neurons were present in the olfactory epithelium adjacent to the basal region. Following days 1 and 3 after nerve transection, no expression of c-jun protein was seen in neurons of the epithelium. On day 9 after transection, neurons in some basal areas indicated expression of c-jun protein. The immunolocalization demonstrated that p53 protein was present in some neurons located on the upper part of the olfactory epithelium. In contrast, an abundance of neurons expressing p53 protein was evident in the olfactory epithelium 1 and 3 days after nerve transection, indicating more cell deaths.(ABSTRACT TRUNCATED AT 250 WORDS)
在正常生理条件下，嗅觉感觉神经元会持续更新。受损的嗅觉感觉神经元也是可替换的。在本研究中，我们通过使用多克隆或单克隆抗嗅觉标记蛋白（OMP）、抗c-jun蛋白和抗p53蛋白抗体进行免疫组织化学染色，研究了大鼠嗅觉上皮神经横断后感觉神经元的细胞分化和生长情况。OMP仅在嗅觉感觉神经元中发现，而c-jun作为一种转录因子发挥作用。p53蛋白作为与DNA损伤诱导的凋亡途径相关的细胞增殖负调节因子。用抗OMP抗体孵育的嗅觉上皮切片显示上皮中成熟神经元和轴突的染色。神经横断导致用OMP标记的神经元显著减少。术后第9天，我们的研究表明有一些恢复，表达OMP的神经元数量增加。在没有神经损伤的对照动物中，c-jun蛋白免疫反应性神经元存在于靠近基底区域的嗅觉上皮中。神经横断后第1天和第3天，上皮神经元中未见c-jun蛋白表达。横断后第9天，一些基底区域的神经元显示c-jun蛋白表达。免疫定位显示p53蛋白存在于位于嗅觉上皮上部的一些神经元中。相反，在神经横断后1天和3天，嗅觉上皮中大量表达p53蛋白的神经元明显可见，表明细胞死亡更多。（摘要截取自250字）
