Guillot Valls M D, Hernández Gil de Tejada T, Martínez-Soriano F
Morphological Sciences Department, Faculty of Medicine and Odontology, Valencia University, Spain.
Histol Histopathol. 1995 Apr;10(2):351-8.
Thirty-six male Wistar rats (225g average b.w.) were studied after direct He-Ne laser irradiation (632.8 nm, 5 mW) of the surgically-exposed pineal gland. Total irradiation time was 5 minutes, with rest intervals of 1 minute for every minute of irradiation. The animals were sacrificed in groups of 4 (controls, irradiated animals and sham-operated rats--i.e., craniotomy without irradiation) on days 3, 7 and 10 postirradiation. A significant increase was seen (p < 0.0001) in the medullary and cortical karyometric indices of the pineal body in all experimental, control and sham-operated animals. The increase was greatest (over 50%) on day 3 postirradiation among the experimental animals, with respect to the control and sham-operated groups. A progressive and significant decrease in karyometric index (p < 0.05) was in turn observed on days 7 and 10 postirradiation, although the values remained higher than among the controls. Ultrastructurally, there were cytoplasmic signs of an increase in metabolic activity in the experimental animals on days 3 and 7, followed by a decrease in activity by day 10 with the appearance of numerous lipid droplets, pericanallicular dark cells and mesoglial cells. We suggest that laser irradiation stimulates cortical and medullary pinealocytes, followed by a decreased effect at day 10 postirradiation. The effect of laser light is in turn determined by experimental action and the duration of exposure.
对36只雄性Wistar大鼠（平均体重225克）进行研究，对手术暴露的松果体进行直接氦氖激光照射（632.8纳米，5毫瓦）。总照射时间为5分钟，每照射1分钟休息1分钟。在照射后第3、7和10天，将动物按每组4只（对照组、照射组动物和假手术大鼠，即开颅但不照射）处死。在所有实验动物、对照动物和假手术动物中，松果体髓质和皮质的核仁测量指数均显著增加（p<0.0001）。与对照组和假手术组相比，实验动物在照射后第3天的增加最为显著（超过50%）。在照射后第7天和第10天，核仁测量指数依次出现渐进性显著下降（p<0.05），尽管其值仍高于对照组。超微结构上，实验动物在第3天和第7天出现代谢活性增加的细胞质迹象，到第10天活性下降，出现大量脂滴、管周暗细胞和中神经胶质细胞。我们认为激光照射刺激了松果体皮质和髓质的松果体细胞，随后在照射后第10天效果减弱。激光的效果又由实验作用和暴露持续时间决定。
