Martinez Soriano F, Ochando R, Hernandez Gil de Tejada T, Cimas C
Morphological Sciences Department, Valencia University, Spain.
J Hirnforsch. 1990;31(3):289-96.
A study is made of the ultrastructure and variations in pinealocyte caryometric indices in 45 male albino rats (240 +/- 40 gm). Fifteen animals were subjected to exposed pineal body irradiation for 6 continuous minutes with soft He-Ne laser light. The irradiated rats were sacrificed in groups of five 3, 6 and 15 days post-irradiation. The control and sham-operated (craniotomy without irradiation) groups consisted of the same number of animals as the experimental groups. The results show a significant decrease in indices (p less than 0.0005) in all experimental groups in relation to the control and sham-operated animals, although in terms of the latter p for the three days post-irradiation group was 0.002. A progressive and significant decrease (p less than 0.002) was also observed between the experimental groups after 3 and 15 days and after 6 and 15 days, but not between the experimental groups after 3 and 6 days. Ultrastructurally, there were signs of secretory activity increase in the groups after 3 and 6 days, and decrease in such activity after 15 days--along with the appearance of cells of mesoglial lineage. A significant increase was observed in the number of peri-canalicular dark cells. The results are discussed and compared with those of other authors and different light energies.
对45只雄性白化大鼠（体重240±40克）的松果体细胞的超微结构和核测量指数变化进行了研究。15只动物用软氦氖激光对松果体进行连续6分钟的照射。照射后的大鼠在照射后3天、6天和15天按每组5只进行处死。对照组和假手术组（开颅但未照射）的动物数量与实验组相同。结果显示，与对照组和假手术组动物相比，所有实验组的指数均显著下降（p<0.0005），不过就假手术组而言，照射后3天组的p值为0.002。在照射后3天与15天之间以及6天与15天之间的实验组之间也观察到了逐渐且显著的下降（p<0.002），但在照射后3天与6天的实验组之间未观察到。在超微结构上，照射后3天和6天的组有分泌活动增加的迹象，而15天后这种活动减少，同时出现了中胶质谱系的细胞。观察到管周暗细胞数量显著增加。对结果进行了讨论，并与其他作者的结果以及不同光能进行了比较。
