Kapteyn J C, Montijn R C, Dijkgraaf G J, Van den Ende H, Klis F M
Institute of Molecular Cell Biology, BioCentrum Amsterdam, University of Amsterdam, The Netherlands.
J Bacteriol. 1995 Jul;177(13):3788-92. doi: 10.1128/jb.177.13.3788-3792.1995.
Yeast and hyphal walls of Candida albicans were extracted with sodium dodecyl sulfate (SDS). Some of the extracted proteins reacted with a specific beta-1,6-glucan antiserum but not with a beta-1,3-glucan antiserum. They lost their beta-1,6-glucan epitope after treatment with ice-cold aqueous hydrofluoric acid, suggesting that beta-1,6-glucan was linked to the protein through a phosphodiester bridge. When yeast and hyphal walls extracted with SDS were subsequently extracted with a pure beta-1,3-glucanase, several mannoproteins that were recognized by both the beta-1,6-glucan antiserum and the beta-1,3-glucan antiserum were released. Both epitopes were sensitive to aqueous hydrofluoric acid treatment, suggesting that beta-1,3-glucan and beta-1,6-glucan are linked to proteins by phosphodiester linkages. The possible role of beta-glucans in the retention of cell wall proteins is discussed.
白色念珠菌的酵母型和菌丝型细胞壁用十二烷基硫酸钠（SDS）进行提取。一些提取出的蛋白质能与特异性β-1,6-葡聚糖抗血清发生反应，但不与β-1,3-葡聚糖抗血清反应。用冰冷的氢氟酸处理后，它们失去了β-1,6-葡聚糖表位，这表明β-1,6-葡聚糖通过磷酸二酯桥与蛋白质相连。当用SDS提取的酵母型和菌丝型细胞壁随后用纯β-1,3-葡聚糖酶提取时，几种能被β-1,6-葡聚糖抗血清和β-1,3-葡聚糖抗血清识别的甘露糖蛋白被释放出来。两种表位都对氢氟酸水溶液处理敏感，这表明β-1,3-葡聚糖和β-1,6-葡聚糖通过磷酸二酯键与蛋白质相连。本文讨论了β-葡聚糖在细胞壁蛋白保留中的可能作用。
