Sanjuán R, Zueco J, Stock R, Font de Mora J, Sentandreu R
Departamento de Microbiología, Facultat de Farmàcia, Universitat de València, Burjassot, Spain.
Microbiology (Reading). 1995 Jul;141 ( Pt 7):1545-51. doi: 10.1099/13500872-141-7-1545.
The use of a novel monoclonal antibody (mAb) that reacts with (1,6)-beta-glucan has permitted the study of the different covalent linkages between glucan and mannoproteins in the cell wall of Candida albicans. The mAb JRR1 was originally raised by immunization with Zymolyase extracts from C. albicans cell walls, but it soon became apparent that it reacted with a (1,6)-beta-glucan epitope. By using this antibody, we show the existence of glucan-mannoprotein complexes between the (1,6)-beta-glucan epitope recognized by the antibody and cell wall mannoproteins. The topology of the (1,6)-beta-glucan in the cell wall of C. albicans has also been studied.
一种与（1,6）-β-葡聚糖发生反应的新型单克隆抗体（mAb）的应用，使得对白色念珠菌细胞壁中葡聚糖和甘露糖蛋白之间不同共价连接的研究成为可能。单克隆抗体JRR1最初是通过用白色念珠菌细胞壁的溶菌酶提取物免疫制备的，但很快就发现它与一个（1,6）-β-葡聚糖表位发生反应。通过使用这种抗体，我们证明了在抗体识别的（1,6）-β-葡聚糖表位与细胞壁甘露糖蛋白之间存在葡聚糖-甘露糖蛋白复合物。我们还研究了白色念珠菌细胞壁中（1,6）-β-葡聚糖的拓扑结构。
