Ohtoshi T, Takizawa H, Sakamaki C, Ishii A, Hirai K, Morita Y, Ito K, Ohta K, Mano K, Suzuki S
Department of Medicine and Physical Therapy, Faculty of Medicine, University of Tokyo, Japan.
Nihon Kyobu Shikkan Gakkai Zasshi. 1994 Dec;32 Suppl:65-72.
There is increasing evidence that airway epithelial cells play an active role in allergic inflammation, including bronchial asthma. We showed that human airway epithelial cells in culture release GM-CSF, G-CSF, M-CSF, IL-6, and IL-8, using a serum-free culture system. These cytokines are known to modulate the bioactivities of inflammatory cells that accumulate at the site of inflammation. Among them, GM-CSF, IL-8, or both may be important because they influence the bioactivities of eosinophils, which are characteristic of allergic inflammation. Here we report on the effects of air pollutants such as suspended particulate matter and diesel exhaust particulates on release of cytokines from airway epithelial cells. All air pollutants we tested stimulated epithelial cells to release GM-CSF. These results suggest that one cause of the recent increase in the prevalence of allergic disorders is direct stimulation of airway epithelial cells by air-pollutants. Furthermore, anti-inflammatory agents such as steroids and anti-allergic drugs were found to suppress the release of GM-CSF from airway epithelial cells in vitro.
越来越多的证据表明，气道上皮细胞在包括支气管哮喘在内的过敏性炎症中发挥着积极作用。我们利用无血清培养系统表明，培养的人气道上皮细胞会释放粒细胞-巨噬细胞集落刺激因子（GM-CSF）、粒细胞集落刺激因子（G-CSF）、巨噬细胞集落刺激因子（M-CSF）、白细胞介素-6（IL-6）和白细胞介素-8（IL-8）。已知这些细胞因子可调节在炎症部位聚集的炎症细胞的生物活性。其中，GM-CSF、IL-8或两者可能很重要，因为它们会影响嗜酸性粒细胞的生物活性，而嗜酸性粒细胞是过敏性炎症的特征。在此，我们报告悬浮颗粒物和柴油废气颗粒物等空气污染物对气道上皮细胞细胞因子释放的影响。我们测试的所有空气污染物均刺激上皮细胞释放GM-CSF。这些结果表明，过敏性疾病近期患病率上升的一个原因是空气污染物对气道上皮细胞的直接刺激。此外，发现类固醇和抗过敏药物等抗炎剂在体外可抑制气道上皮细胞释放GM-CSF。
