Sreeramulu G, Singh N K
Central Food Technological Research Institute, Mysore, India.
Electrophoresis. 1995 Mar;16(3):362-5. doi: 10.1002/elps.1150160162.
A novel method for destaining of polyacrylamide gels, stained with Coomassie Brilliant Blue R-250, is described, based on the use of 0.5 M NaCl in water as the destainer, requiring only 2-3 h. Concentrated (> 2 M) or dilute (< 0.1 M) salt solutions were unsuitable. The method affords the advantage that expensive organic solvents, such as methanol, acetic acid or trichloroacetic acid, are not needed. Furthermore, salt destaining results in darker purple-blue protein bands as compared to the pale blue color with e.g. methanol/acetic acid destaining.
本文描述了一种用于对用考马斯亮蓝R-250染色的聚丙烯酰胺凝胶进行脱色的新方法，该方法基于使用0.5M氯化钠水溶液作为脱色剂，只需2-3小时。浓（>2M）或稀（<0.1M）盐溶液均不合适。该方法的优点是不需要昂贵的有机溶剂，如甲醇、乙酸或三氯乙酸。此外，与例如用甲醇/乙酸脱色产生的浅蓝色相比，盐脱色产生的蛋白质条带呈更深的紫蓝色。
