Zhao Z, Wahl J H, Udseth H R, Hofstadler S A, Fuciarelli A F, Smith R D
Chemical Sciences Department, Pacific Northwest Laboratory, Richland, WA 99352, USA.
Electrophoresis. 1995 Mar;16(3):389-95. doi: 10.1002/elps.1150160165.
Capillary zone electrophoresis (CZE) and reversed anionic capillary isotachophoresis (CITP) conditions have been developed for the separation of mixtures comprised of monophosphate nucleosides, pyridine and flavin dinucleotides, and monophosphate dinucleosides. Results for the on-line coupling of CZE and CITP with electrospray ionization mass spectrometry (MS) are presented. CITP-tandem MS is utilized to provide both molecular weight and structural information of monophosphate dinucleotides. The fragmentation pattern of dinucleotides in the low collision energy range is described. The resulting mass spectra are readily interpreted in terms of dinucleotide structures. These results demonstrate the new capability for applications for the study of DNA and RNA.
已开发出毛细管区带电泳（CZE）和反向阴离子毛细管等速电泳（CITP）条件，用于分离由单磷酸核苷、吡啶和黄素二核苷酸以及单磷酸二核苷组成的混合物。本文展示了CZE和CITP与电喷雾电离质谱（MS）在线联用的结果。利用CITP串联质谱来提供单磷酸二核苷的分子量和结构信息。描述了二核苷酸在低碰撞能量范围内的裂解模式。所得质谱图可根据二核苷酸结构轻松解读。这些结果证明了其在DNA和RNA研究应用方面的新能力。
