Zhao Z, Fuciarelli A F, Smith R D
Merck Research Laboratories, West Point, PA, USA.
J Capillary Electrophor. 1996 Mar-Apr;3(2):111-6.
Analysis of trace levels of DNA damage as a biomarker of human exposure to radiation levels represents a significant challenge. Capillary isotachophoresis (CITP) is well suited for this application due to its high separation efficiency, large sample loading capacity, and relative ease of interfacing with highly specific and sensitive mass spectrometric detectors. This study describes the use of reversed anionic CITP coupled on-line with electrospray ionization-mass spectrometry (ESI-MS) for the identification and characterization of small quantities of radiation induced damage to deoxynucleotide monophosphates. CITP with ESI-tandem MS also provides structural information on the nature of the damage in addition to the molecular weight. This approach has the attraction of allowing the direct on-line determination of damaged nucleotides, as well as the free bases and nucleosides, and avoids the need for sample hydrolysis and/or derivatization.
分析痕量水平的DNA损伤作为人类暴露于辐射水平的生物标志物是一项重大挑战。毛细管等速电泳（CITP）由于其高分离效率、大样品加载量以及相对易于与高特异性和灵敏的质谱检测器联用，非常适合此应用。本研究描述了使用反向阴离子CITP与电喷雾电离质谱（ESI-MS）在线联用，用于鉴定和表征少量辐射诱导的脱氧单磷酸核苷酸损伤。带有ESI串联质谱的CITP除了提供分子量信息外，还能提供损伤性质的结构信息。这种方法的吸引力在于能够直接在线测定受损核苷酸以及游离碱基和核苷，并且无需进行样品水解和/或衍生化。
