Morariu V V, Kiricuta I C, Hazlewood C F
Physiol Chem Phys. 1978;10(6):517-24.
The proton linewidth and the integral of the absorption signal of water in rat kidney tissue have been measured at sub-zero temperatures. The water, fresh or stored at +4 degrees C for 24--48 h, shows a supercooling effect down to -10 degrees C to -14 degrees C. At -20 degrees C, 10--20% of the total tissue water remains unfrozen. The supercooling effect is diminished by prior storage at positive temperatures but completely eliminated by storage at several degrees below 0 degrees C. This suggests that the freezing behavior of tissue water varies with change in the physiological state of the tissue. Tissue samples stored at positive temperatures were observed to be more vulnerable than fresh tissue to freezing damage. Storage at several degrees below 0 degrees C for the same period of time appeared to be even more damaging.
已在零下温度下测量了大鼠肾组织中水的质子线宽和吸收信号积分。新鲜的水或在4℃储存24 - 48小时的水，会出现过冷效应，直至 - 10℃至 - 14℃。在 - 20℃时，总组织水的10% - 20%仍未冻结。正温度下的预先储存会减弱过冷效应，但在低于0℃几度的温度下储存则会完全消除过冷效应。这表明组织水的冻结行为随组织生理状态的变化而变化。观察到在正温度下储存的组织样本比新鲜组织更容易受到冷冻损伤。在低于0℃几度的温度下储存相同时间似乎更具破坏性。
