Isozaki K, Hirota S, Nakama A, Miyagawa J, Shinomura Y, Xu Z, Nomura S, Kitamura Y
Department of Pathology, Osaka University Medical School, Suita, Japan.
Gastroenterology. 1995 Aug;109(2):456-64. doi: 10.1016/0016-5085(95)90333-x.
BACKGROUND & AIMS: Interstitial cells of Cajal (ICCs) are believed to initiate the basic contractile activity of the gastrointestinal tract. Because ICCs in the intestine of mice express c-kit receptor tyrosine kinase and because rats are more commonly used than mice for pathophysiological investigations of the gastrointestinal tract, the number of the c-kit messenger RNA-expressing cells was compared with gastrointestinal movement in rats.
The c-kit messenger RNA-expressing cells were detected by in situ hybridization. The autonomous contraction of excised segments of the ileum was recorded. The function of the pyloric sphincter was evaluated by measuring the content of bile acids in the stomach.
The c-kit messenger RNA-expressing cells were not detectable in the stomach of Ws/Ws mutant rats with a small deletion at the tyrosine kinase domain of c-kit, and the number of c-kit messenger RNA-expressing cells decreased to 7% that of normal control rats in the ileum of Ws/Ws rats. The contractile activity of the ileum was apparently impaired, and the content of bile acids in the stomach was significantly increased in Ws/Ws rats.
The abnormalities in the ileal movement and pyloric sphincter function in Ws/Ws rats were attributable to the deficiency of c-kit messenger RNA-expressing cells.
人们认为， Cajal间质细胞（ICCs）启动胃肠道的基本收缩活动。由于小鼠肠道中的ICCs表达c-kit受体酪氨酸激酶，并且由于在胃肠道病理生理学研究中大鼠比小鼠更常用，因此将表达c-kit信使核糖核酸的细胞数量与大鼠的胃肠运动进行了比较。
通过原位杂交检测表达c-kit信使核糖核酸的细胞。记录回肠切除段的自主收缩。通过测量胃中胆汁酸的含量来评估幽门括约肌的功能。
在c-kit酪氨酸激酶结构域有小缺失的Ws/Ws突变大鼠的胃中未检测到表达c-kit信使核糖核酸的细胞，并且在Ws/Ws大鼠的回肠中，表达c-kit信使核糖核酸的细胞数量降至正常对照大鼠的7%。Ws/Ws大鼠回肠的收缩活动明显受损，胃中胆汁酸的含量显著增加。
Ws/Ws大鼠回肠运动和幽门括约肌功能异常归因于表达c-kit信使核糖核酸的细胞缺乏。
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