电针足三里通过SDF-1/CXCR4和mSCF/Kit-ETV1通路增加糖尿病小鼠胃中骨髓源性Cajal间质细胞

Electroacupuncture at ST36 Increases Bone Marrow-Derived Interstitial Cells of Cajal via the SDF-1/CXCR4 and mSCF/Kit-ETV1 Pathways in the Stomach of Diabetic Mice.

作者信息

Zhao Jiao, An Jing, Liu Shi

机构信息

Division of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

出版信息

Evid Based Complement Alternat Med. 2018 Jan 23;2018:7878053. doi: 10.1155/2018/7878053. eCollection 2018.

BACKGROUND

The loss of interstitial cells of Cajal (ICC) is observed in diabetic gastroparesis. Electroacupuncture (EA) maintains ICC networks, but the effects and mechanisms of EA on ICC of bone marrow derivation in the stomach have not been investigated.

METHODS

C57BL/6 mice were randomized into six groups: control, diabetic (DM), bone marrow transplantation (BMT) + DM, BMT + DM + sham EA (SEA), BMT + DM + low-frequency EA (LEA), and BMT + DM + high-frequency (HEA). c-KitGFP cells in the stomach were detected by immunofluorescence staining. Western blotting and qRT-PCR were employed to determine c-Kit, GFP, SDF-1, CXCR4, mSCF, pERK, and ETV1 expression.

RESULTS

(1) c-KitGFP cells were elevated in the BMT + DM + LEA and HEA groups. (2) The mRNA and protein levels of GFP, SDF-1, and CXCR4 were increased in the BMT + DM + LEA and BMT + DM + HEA groups. (3) The mRNA and protein levels of mSCF, c-Kit, pERK, and ETV1 were significantly reduced in the DM group but markedly elevated in the BMT + DM + LEA and HEA groups.

CONCLUSION

EA at ST36 increases bone marrow-derived ICC in the stomach of diabetic mice via the SDF-1/CXCR4 and mSCF/c-Kit-ETV1 pathways.

背景

在糖尿病胃轻瘫中观察到Cajal间质细胞（ICC）的丢失。电针（EA）可维持ICC网络，但EA对胃中骨髓源性ICC的影响及机制尚未得到研究。

方法

将C57BL/6小鼠随机分为六组：对照组、糖尿病组（DM）、骨髓移植（BMT）+DM组、BMT+DM+假电针（SEA）组、BMT+DM+低频电针（LEA）组和BMT+DM+高频电针（HEA）组。通过免疫荧光染色检测胃中的c-KitGFP细胞。采用蛋白质印迹法和qRT-PCR法测定c-Kit、GFP、SDF-1、CXCR4、mSCF、pERK和ETV1的表达。

结果

（1）BMT+DM+LEA组和HEA组中的c-KitGFP细胞增多。（2）BMT+DM+LEA组和BMT+DM+HEA组中GFP、SDF-1和CXCR4的mRNA及蛋白水平升高。（3）DM组中mSCF、c-Kit、pERK和ETV1的mRNA及蛋白水平显著降低，但在BMT+DM+LEA组和HEA组中明显升高。