用聚合酶链反应-脱氧核糖核酸探针法鉴定胎儿弯曲杆菌
Identification of Campylobacter fetus by PCR-DNA probe method.
作者信息
Blom K, Patton C M, Nicholson M A, Swaminathan B
机构信息
Foodborne and Diarrheal Diseases Branch, National Center for Infectious Diseases, Atlanta, Georgia 30333, USA.
出版信息
J Clin Microbiol. 1995 May;33(5):1360-2. doi: 10.1128/jcm.33.5.1360-1362.1995.
Abstract
A PCR method for rapid identification of Campylobacter fetus subsp. fetus was evaluated. A fragment of the gene coding for 16S rRNA was amplified from crude cell lysates of 18 C. fetus strains and 30 strains representing other Campylobacter species and subspecies. The amplicons were probed by dot blot hybridization with a digoxigenin-labeled C. fetus-specific oligonucleotide probe. The probe reacted only with C. fetus subsp. fetus and C. fetus subsp. venerealis and may be useful for rapid identification in clinical laboratories.
摘要
评估了一种用于快速鉴定胎儿弯曲菌胎儿亚种的聚合酶链反应（PCR）方法。从18株胎儿弯曲菌菌株以及代表其他弯曲菌属物种和亚种的30株菌株的粗细胞裂解物中扩增出编码16S核糖体RNA（rRNA）的基因片段。通过用洋地黄毒苷标记的胎儿弯曲菌特异性寡核苷酸探针进行斑点印迹杂交来检测扩增产物。该探针仅与胎儿弯曲菌胎儿亚种和胎儿弯曲菌性病亚种发生反应，可能有助于临床实验室的快速鉴定。
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