Misúrová E, Fedorocko P
Department of Cellular and Molecular Biology, Faculty of Science, P.J. Safárik University, Kosice, Slovakia.
Neoplasma. 1995;42(2):63-8.
Effects of liposomal muramyl tripeptide phosphatidylethanolamine (MTP-PE/MLV) on nucleic acid content, and on DNA synthesis and cellularity in the blood and haemopoietic tissues of mice irradiated with a dose of 6 Gy gamma radiation were studied. It was found that injection of MTP-PE/MLV alone (200 micrograms per mouse; i.p.) resulted in a mild decrease in DNA content in the bone marrow as consequence of cellularity diminution in the femur. At the same time, incorporation of 3H-thymidine into bone marrow DNA increased in comparison to the non-stimulated mice. On the basis of simultaneous increase in RNA and DNA concentration in the blood we assume that the changes of nucleic acids and cellularity in the bone marrow of MTP-PE/MLV treated mice are related to the higher release of nuclear cells into the circulation. In mice irradiated 24 h after MTP-PE/MLV injection the decrease in nucleic acid content was similar to that in unprotected mice within the first three days after irradiation. However, during following days, the recovery of RNA and DNA content and cellularity in the bone marrow and blood proceeded at a much faster rate in mice protected by MTP-PE/MLV as compared to unprotected animals. The increase in DNA synthesis in the bone marrow of protected mice preceded the DNA content recovery. In the spleen and the thymus, no effect of liposomal MTP-PE was found.
研究了脂质体胞壁酰三肽磷脂酰乙醇胺（MTP - PE/MLV）对接受6 Gyγ射线照射的小鼠血液和造血组织中核酸含量、DNA合成及细胞数量的影响。发现单独注射MTP - PE/MLV（每只小鼠200微克；腹腔注射）会导致骨髓中DNA含量略有下降，这是由于股骨中细胞数量减少所致。同时，与未受刺激的小鼠相比，骨髓DNA中3H - 胸苷的掺入量增加。基于血液中RNA和DNA浓度同时升高，我们推测经MTP - PE/MLV处理的小鼠骨髓中核酸和细胞数量的变化与更多的核细胞释放到循环系统有关。在注射MTP - PE/MLV后24小时接受照射的小鼠中，核酸含量的下降在照射后的头三天与未受保护的小鼠相似。然而，在随后的几天里，与未受保护的动物相比，经MTP - PE/MLV保护的小鼠骨髓和血液中RNA和DNA含量以及细胞数量的恢复速度要快得多。受保护小鼠骨髓中DNA合成的增加先于DNA含量的恢复。在脾脏和胸腺中，未发现脂质体MTP - PE有作用。
