Macková N O, Fedorocko P
Department of Cellular and Molecular Biology, Faculty of Science, Safarik University, Kosice, Slovak Republic.
Physiol Res. 2002;51(5):511-21.
The effects of liposomal muramyl tripeptide phosphatidylethanolamine (MTP-PE/MLV, radioprotective immunomodulator; 10 mg/kg) and indomethacin (INDO, inhibitor of prostaglandin production; 2 mg/kg) on post-irradiation recovery of hematopoietic functions in mice were investigated. Two agents with distinct radioprotective mechanisms were administered alone or in combination 24 h and 3 h before exposure to 7 Gy (60)Co radiation. In the post-irradiation period (3-14 days) combined pre-treatment of mice accelerated recovery of bone marrow cellularity, weight of spleen and myelopoietic and erythropoietic activity in both hematopoietic organs, compared to treatment with MTP-PE/MLV or indomethacin alone. In the peripheral blood, improved radioprotective effects of combined drug administration were found in the recovery of reticulocytes and platelet count. No further significant differences in the recovery of leukocyte count were observed in the examined groups until post-irradiation day 14. Within the first 3-6 post-irradiation days, the bone marrow and peripheral blood smears of mice pre-treated with indomethacin alone or its combination with MTP-PE/MLV more frequently featured blast cells and large cells with abundant cytoplasm which could be considered the hematopoietic stem cells.
研究了脂质体胞壁酰三肽磷脂酰乙醇胺（MTP - PE/MLV，辐射防护免疫调节剂；10毫克/千克）和吲哚美辛（INDO，前列腺素生成抑制剂；2毫克/千克）对小鼠辐射后造血功能恢复的影响。在暴露于7 Gy（60）Co辐射前24小时和3小时，单独或联合给予两种具有不同辐射防护机制的药物。与单独使用MTP - PE/MLV或吲哚美辛治疗相比，在辐射后时期（3 - 14天），联合预处理小鼠可加速两个造血器官的骨髓细胞密度、脾脏重量以及骨髓生成和红细胞生成活性的恢复。在外周血中，联合给药在网织红细胞和血小板计数的恢复方面显示出更好的辐射防护效果。在照射后第14天之前，各检测组白细胞计数的恢复未观察到进一步的显著差异。在照射后的前3 - 6天内，单独用吲哚美辛或其与MTP - PE/MLV联合预处理的小鼠的骨髓和外周血涂片更频繁地出现原始细胞和具有丰富细胞质的大细胞，这些细胞可被视为造血干细胞。
