Sieling P A, Chatterjee D, Porcelli S A, Prigozy T I, Mazzaccaro R J, Soriano T, Bloom B R, Brenner M B, Kronenberg M, Brennan P J
Division of Dermatology, University of California at Los Angeles (UCLA) School of Medicine 90095, USA.
Science. 1995 Jul 14;269(5221):227-30. doi: 10.1126/science.7542404.
It has long been the paradigm that T cells recognize peptide antigens presented by major histocompatibility complex (MHC) molecules. However, nonpeptide antigens can be presented to T cells by human CD1b molecules, which are not encoded by the MHC. A major class of microbial antigens associated with pathogenicity are lipoglycans. It is shown here that human CD1b presents the defined mycobacterial lipoglycan lipoarabinomannan (LAM) to alpha beta T cell receptor-bearing lymphocytes. Presentation of these lipoglycan antigens required internalization and endosomal acidification. The T cell recognition required mannosides with alpha(1-->2) linkages and a phosphotidylinositol unit. T cells activated by LAM produced interferon gamma and were cytolytic. Thus, an important class of microbial molecules, the lipoglycans, is a part of the universe of foreign antigens recognized by human T cells.
长期以来的范式是，T细胞识别由主要组织相容性复合体（MHC）分子呈递的肽抗原。然而，非肽抗原可由人类CD1b分子呈递给T细胞，而CD1b分子并非由MHC编码。一类与致病性相关的主要微生物抗原是脂聚糖。本文表明，人类CD1b将确定的分枝杆菌脂聚糖脂阿拉伯甘露聚糖（LAM）呈递给携带αβT细胞受体的淋巴细胞。这些脂聚糖抗原的呈递需要内化和内体酸化。T细胞识别需要具有α（1→2）连接的甘露糖苷和一个磷脂酰肌醇单元。被LAM激活的T细胞产生干扰素γ并具有细胞毒性。因此，一类重要的微生物分子——脂聚糖，是人类T细胞识别的外来抗原范畴的一部分。
