Głabiński A, Mirecka M, Pokoca L
Department of Neurology, Medical University, Lodz, Poland.
Acta Neurol Scand. 1995 Apr;91(4):276-9. doi: 10.1111/j.1600-0404.1995.tb07004.x.
The cytotoxic cytokines tumor necrosis factor alpha (TNF-alpha) and lymphotoxin (LT) possess toxic activity against myelin and/or oligodendrocytes in vitro. Multiple sclerosis (MS) plaques within the central nervous system (CNS) are infiltrated by peripheral blood mononuclear cells (PBMC). In this study the production of TNF-alpha and LT by PBMC in active MS were measured. PBMC were isolated from the blood of MS patients in relapse and also patients with other neurological diseases (OND) and healthy controls (HC). Isolated cells were cultured unstimulated or stimulated with phytohemagglutinin A (PHA), lipopolysaccharide (LPS) and myelin basic protein (MBP)--a hypothetical autoantigen for MS. Cytokine production was assessed using ELISA method. In the MS group, PBMC without stimulation as well as after stimulation with MBP displayed a significantly increased production of TNF-alpha. LT production was similar in MS and control groups. These results suggest that TNF-alpha but not LT is overproduced by PBMC during MS relapse.
细胞毒性细胞因子肿瘤坏死因子α（TNF-α）和淋巴毒素（LT）在体外对髓磷脂和/或少突胶质细胞具有毒性活性。中枢神经系统（CNS）内的多发性硬化症（MS）斑块被外周血单核细胞（PBMC）浸润。在本研究中，对活动性MS患者外周血单核细胞产生TNF-α和LT的情况进行了检测。从复发期MS患者以及患有其他神经系统疾病（OND）的患者和健康对照（HC）的血液中分离出PBMC。将分离出的细胞在未刺激或用植物血凝素A（PHA）、脂多糖（LPS）和髓磷脂碱性蛋白（MBP，一种假设的MS自身抗原）刺激的情况下进行培养。使用酶联免疫吸附测定（ELISA）方法评估细胞因子的产生。在MS组中，未刺激的PBMC以及用MBP刺激后的PBMC产生的TNF-α显著增加。MS组和对照组的LT产生情况相似。这些结果表明，在MS复发期间，PBMC过度产生的是TNF-α而非LT。
