Formigli L, Manneschi L I, Adembri C, Orlandini S Z, Pratesi C, Novelli G P
Department of Human Anatomy and Histology, University of Florence, Italy.
Ultrastruct Pathol. 1995 May-Jun;19(3):193-200. doi: 10.3109/01913129509064221.
This work was undertaken to assess the role of endothelial E-selectin in the development of neutrophil accumulation into the ischemic and reperfused human skeletal muscle and eventually in the genesis of ischemia-reperfusion syndrome. Twelve patients affected by abdominal aortic aneurysm who were undergoing reconstructive vascular surgery were studied. Muscle biopsies from the right femoral quadriceps were taken (1) immediately after anesthesia, as control samples, (2) before declamping the aorta, as ischemic samples, and (3) 30 minutes after reperfusion and then processed for immunohistochemical and ultrastructural analysis. Immunohistochemistry revealed a strong positive reaction for E-selectin on the venular endothelium during ischemia and reperfusion. Ultrastructural investigation showed that reactivity for E-selectin matched neutrophil accumulation of the skeletal muscle tissue. This phenomenon was dependent upon a complex series of events that included neutrophil adhesion to the inner surface of the postcapillary venules, passage through endothelial intercellular junctions, and migration distally into the interstitial spaces of the skeletal muscle tissue. Neutrophil tissue infiltration was also associated with ultrastructural signs of tissue damage at reperfusion. This is in agreement with accumulating evidence indicating a role for tissue infiltrating neutrophils in the genesis of toxic O2 free radicals. Our data suggest that E-selectin expression on the vascular endothelium of human skeletal muscle may represent a key regulatory point in the process of neutrophil tissue accumulation and indicate an active role for the venular endothelium in the development of human ischemia-reperfusion syndrome.
本研究旨在评估内皮细胞E选择素在中性粒细胞积聚至缺血再灌注的人体骨骼肌过程中的作用，以及最终在缺血再灌注综合征发生中的作用。对12例接受血管重建手术的腹主动脉瘤患者进行了研究。取右侧股四头肌的肌肉活检组织：(1)麻醉后立即取材作为对照样本；(2)主动脉夹闭前取材作为缺血样本；(3)再灌注30分钟后取材，然后进行免疫组织化学和超微结构分析。免疫组织化学显示，在缺血和再灌注期间，小静脉内皮细胞上的E选择素呈强阳性反应。超微结构研究表明，E选择素的反应性与骨骼肌组织中的中性粒细胞积聚相匹配。这种现象依赖于一系列复杂事件，包括中性粒细胞黏附于毛细血管后小静脉内表面、穿过内皮细胞间连接以及向远端迁移至骨骼肌组织的间隙。中性粒细胞组织浸润还与再灌注时组织损伤的超微结构征象相关。这与越来越多的证据一致，这些证据表明组织浸润的中性粒细胞在毒性氧自由基生成中起作用。我们的数据表明，人体骨骼肌血管内皮细胞上的E选择素表达可能是中性粒细胞组织积聚过程中的关键调节点，并表明小静脉内皮细胞在人体缺血再灌注综合征发生中起积极作用。
