正常妊娠和子痫前期患者血小板中一氧化氮合酶活性的差异

Differential nitric oxide synthase activity in human platelets during normal pregnancy and pre-eclampsia.

Delacrétaz E, de Quay N, Waeber B, Vial Y, Schulz P E, Burnier M, Brunner H R, Bossart H, Schaad N C

Division of Hypertension, University Hospital of Lausanne, Switzerland.

Clin Sci (Lond). 1995 Jun;88(6):607-10. doi: 10.1042/cs0880607.

Abstract

Nitric oxide released from endothelial cells is a potent vasodilator that might play an important role in cardiovascular regulation during pregnancy. Platelets, like endothelial cells, contain a constitutive form of nitric oxide synthase. 2. The present study aimed to measure the activity of this nitric oxide-forming enzyme in normotensive pregnant and non-pregnant women, as well as in women who had developed pre-eclampsia. Nitric oxide synthase activity was measured in the platelets of 21 normotensive pregnant women, 16 non-pregnant women and seven pregnant women who had developed pre-eclampsia. 3. The nitric oxide synthase activity was significantly higher in normotensive pregnant women [36.8 +/- 2.7 pmol h-1 mg-1 of protein (mean +/- SEM), P < 0.001] than in non-pregnant control subjects (16.8 +/- 1.4 pmol h-1 mg-1 of protein) and in women with pre-eclampsia (24.5 +/- 2.1 pmol h-1 mg-1 of protein, P < 0.01). 4. These data suggest that nitric oxide synthesis is increased during normal pregnancy, possibly contributing to the vasodilatation associated with this condition. Nitric oxide generation, however, may be inappropriately low in pregnant women developing pre-eclampsia, thus leading to an enhanced vasoconstriction.

摘要

内皮细胞释放的一氧化氮是一种强效血管舒张剂，可能在孕期心血管调节中发挥重要作用。血小板与内皮细胞一样，含有组成型一氧化氮合酶。2. 本研究旨在测定血压正常的孕妇和非孕妇以及患先兆子痫的女性体内这种生成一氧化氮的酶的活性。测定了21名血压正常的孕妇、16名非孕妇和7名患先兆子痫的孕妇血小板中的一氧化氮合酶活性。3. 血压正常的孕妇体内一氧化氮合酶活性[36.8±2.7皮摩尔·小时⁻¹·毫克⁻¹蛋白质（平均值±标准误），P<0.001]显著高于非孕对照者（16.8±1.4皮摩尔·小时⁻¹·毫克⁻¹蛋白质）和患先兆子痫的女性（24.5±2.1皮摩尔·小时⁻¹·毫克⁻¹蛋白质，P<0.01）。4. 这些数据表明，正常孕期一氧化氮合成增加，这可能有助于与该状态相关的血管舒张。然而，患先兆子痫的孕妇体内一氧化氮生成可能过低，从而导致血管收缩增强。