Baker P N, Davidge S T, Roberts J M
Magee-Womens Research Institute, University of Pittsburgh (Pa) School of Medicine, USA.
Hypertension. 1995 Aug;26(2):244-8. doi: 10.1161/01.hyp.26.2.244.
In preeclampsia, a factor in the maternal circulation alters endothelial function via a reduction in nitric oxide synthesis. We measured the in vitro effects of 2% plasma from women with preeclampsia, compared with 2% plasma from normotensive pregnant women, on cultured endothelial cell nitrite production and nitric oxide synthase activity. On finding differential effects, we measured the effects on cellular viability (assessed by lactate dehydrogenase levels) and performed a time course study. Endothelial cell nitrite production was found to be higher after exposure to plasma from the preeclamptic group than the normotensive pregnant group. The effects of long-term exposure (120 hours) were similar to those of short-term exposure (24 hours). In addition, nitric oxide synthase activity was significantly greater after exposure to preeclamptic plasma than after exposure to normotensive pregnant plasma. No differential effect on cellular viability was found. Contrary to our hypothesis, exposure of endothelial cells to preeclamptic plasma resulted in increased nitric oxide production and nitric oxide synthase activity.
在子痫前期，母体循环中的一种因子通过减少一氧化氮合成来改变内皮功能。我们测定了子痫前期女性2%血浆与血压正常孕妇2%血浆对培养的内皮细胞亚硝酸盐生成和一氧化氮合酶活性的体外影响。在发现差异效应后，我们测定了对细胞活力的影响（通过乳酸脱氢酶水平评估）并进行了时间进程研究。发现暴露于子痫前期组血浆后的内皮细胞亚硝酸盐生成高于血压正常的妊娠组。长期暴露（120小时）的效应与短期暴露（24小时）相似。此外，暴露于子痫前期血浆后的一氧化氮合酶活性显著高于暴露于血压正常孕妇血浆后。未发现对细胞活力有差异效应。与我们的假设相反，内皮细胞暴露于子痫前期血浆导致一氧化氮生成增加和一氧化氮合酶活性增强。
