Thomssen H, Ivanyi J, Espitia C, Arya A, Londei M
Mathilda and Terence Kennedy, Institute of Rheumatology, Sunley Division, Hammersmith, London, UK.
Immunology. 1995 May;85(1):33-40.
Double-negative alpha beta+ T-cell receptor (TCR) human T cells have been reported to recognize antigen in the context of the HLA class I-like (Ib) CD1 complex. In particular, the CD1b molecule has been shown to act as the element of genetic restriction for antigens derived from Mycobacterium tuberculosis. The stenotopic nature of these major histocompatibility complex (MHC) class Ib molecules raised the question of whether the antigenic moiety recognized by CD4-CD8- alpha beta+ TCR T cells was shared by different mycobacteria. We demonstrate here that a CD4-CD8- alpha beta+ TCR T-cell line and three clones raised against M. tuberculosis proliferated following stimulation with soluble extracts from organisms of the M. tuberculosis complex, M. leprae and 10 out of 16 tested isolates of M. avium complex; however, four species of weakly or non-pathogenic mycobacteria were not stimulatory. Furthermore, the M. tuberculosis soluble extract (MTSE)-derived, recognized antigenic moiety proved to be proteinase K resistant and to have a molecular weight greater than 5000 MW, thus it differed from the reported antigenic moiety, recognized by CD4-CD8- gamma delta+ TCR cells. Our results suggest that a common antigenic moiety, presented by CD1b molecules to CD4-CD8- alpha beta+ TCR T cells, is shared by many mycobacterial species. Therefore they raise interest in the question of whether CD4-CD8- alpha beta+ TCR T cells, elicited by M. tuberculosis, may play a role in the natural history of other mycobacterial infections.
据报道，双阴性αβ⁺T细胞受体（TCR）的人类T细胞可在HLA I类样（Ib）CD1复合物的背景下识别抗原。特别是，已证明CD1b分子可作为结核分枝杆菌衍生抗原的遗传限制元件。这些主要组织相容性复合体（MHC）Ib类分子的狭窄特异性引发了一个问题，即CD4⁻CD8⁻αβ⁺TCR T细胞识别的抗原部分是否为不同分枝杆菌所共有。我们在此证明，一条针对结核分枝杆菌产生的CD4⁻CD8⁻αβ⁺TCR T细胞系和三个克隆在用结核分枝杆菌复合群、麻风分枝杆菌以及16株鸟分枝杆菌复合群测试菌株中的10株的可溶性提取物刺激后发生增殖；然而，四种弱致病性或非致病性分枝杆菌无刺激作用。此外，源自结核分枝杆菌可溶性提取物（MTSE）的被识别抗原部分经证明对蛋白酶K具有抗性，且分子量大于5000 MW，因此它与报道的被CD4⁻CD8⁻γδ⁺TCR细胞识别的抗原部分不同。我们的结果表明，由CD1b分子呈递给CD4⁻CD8⁻αβ⁺TCR T细胞的一种共同抗原部分为许多分枝杆菌物种所共有。因此，它们引发了人们对于由结核分枝杆菌引发的CD4⁻CD8⁻αβ⁺TCR T细胞是否可能在其他分枝杆菌感染的自然病程中发挥作用这一问题的兴趣。
