Fairhurst R M, Wang C X, Sieling P A, Modlin R L, Braun J
Molecular Biology Institute, UCLA School of Medicine, Los Angeles, California 90095, USA.
Infect Immun. 1998 Aug;66(8):3523-6. doi: 10.1128/IAI.66.8.3523-3526.1998.
Human CD1 is a family of nonpolymorphic major histocompatibility complex class I-like molecules capable of presenting mycobacterial lipids, including lipoarabinomannan (LAM), to double-negative (DN; CD4(-) CD8(-)) as well as CD8(+) T cells. Structural similarities between LAM and the capsular polysaccharides of gram-negative bacteria led us to consider the latter as candidate CD1 ligands. We derived two CD1-restricted DN T-cell populations which proliferated to Haemophilus influenzae type b (Hib) antigen. One T-cell population also proliferated to proteinase K-treated Hib antigen, suggesting that it recognized a nonpeptide. Our work thus expands the universe of T cell antigens to include nonpeptides distinct from mycobacterial lipids and suggests a potential role for CD1-restricted T cells in immunity to Hib.
人类CD1是一类非多态性的主要组织相容性复合体I类样分子，能够将包括脂阿拉伯甘露聚糖（LAM）在内的分枝杆菌脂质呈递给双阴性（DN；CD4(-) CD8(-)）以及CD8(+) T细胞。LAM与革兰氏阴性菌荚膜多糖之间的结构相似性使我们将后者视为CD1配体的候选物。我们获得了两个受CD1限制的DN T细胞群体，它们对b型流感嗜血杆菌（Hib）抗原产生增殖反应。其中一个T细胞群体对蛋白酶K处理的Hib抗原也有增殖反应，这表明它识别的是一种非肽。因此，我们的工作将T细胞抗原的范围扩展到包括不同于分枝杆菌脂质的非肽，并提示受CD1限制的T细胞在针对Hib的免疫中可能发挥作用。
