Ide A, Hashimoto T, Amagai M, Tanaka M, Nishikawa T
Department of Dermatology, Keio University School of Medicine, Tokyo, Japan.
Exp Dermatol. 1995 Apr;4(2):112-6. doi: 10.1111/j.1600-0625.1995.tb00232.x.
To develop a new method to evaluate autoantibodies in various autoimmune bullous skin diseases, we examined reactivity of bullous pemphigoid (BP) and pemphigus vulgaris (PV) patients' sera with partial bacterial fusion proteins of the 230 kD BP antigen (BPAG1) and PV antigen, respectively, by an enzyme-linked immunosorbent assay (ELISA), and compared the results with those of immunoblotting. We used two fusion proteins derived from the mouse BPAG1 and a fusion protein derived from the amino-terminus (EC1-2) of PV antigen. For both BP and PV sera, the ELISA scores were well correlated with the reactivities on immunoblot assays. The present study indicates that ELISA using recombinant antigen proteins in various autoimmune bullous skin diseases will be a new useful technique to detect the autoantibodies. However, development of recombinant proteins with the entire molecule and correct conformation will be necessary to establish a perfect ELISA system in the future.
为开发一种评估各种自身免疫性大疱性皮肤病中自身抗体的新方法，我们通过酶联免疫吸附测定（ELISA）分别检测了大疱性类天疱疮（BP）和寻常型天疱疮（PV）患者血清与230kD BP抗原（BPAG1）和PV抗原的部分细菌融合蛋白的反应性，并将结果与免疫印迹结果进行比较。我们使用了两种源自小鼠BPAG1的融合蛋白和一种源自PV抗原氨基末端（EC1-2）的融合蛋白。对于BP和PV血清，ELISA评分与免疫印迹分析的反应性高度相关。本研究表明，在各种自身免疫性大疱性皮肤病中使用重组抗原蛋白的ELISA将是检测自身抗体的一种新的有用技术。然而，未来建立完善的ELISA系统需要开发具有完整分子和正确构象的重组蛋白。
