Fristad I, Heyeraas K J, Kvinnsland I H, Jonsson R
Department of Cariology and Endodontics, School of Dentistry, University of Bergen, Norway.
J Histochem Cytochem. 1995 Sep;43(9):871-9. doi: 10.1177/43.9.7543913.
The dental pulp represents a peripheral end-organ deprived of a collateral nerve supply. After inferior alveolar nerve (IAN) axotomy, rat molar pulp is denervated over a period of at least 6 days. Therefore, rat molar pulp was used as an experimental model to study the effect of sensory nerve fibers on influx of immunocompetent cells after dentinal injury. In the present study we performed a quantitative analysis of CD43+, CD4+, CD11b+, and I-A antigen-expressing cells subjacent to dentinal cavities in denervated and innervated first mandibular molars. For visualization of nerve fibers, antibodies to protein gene product (PGP) 9.5, the sensory neuropeptides substance P (SP) and calcitonin gene-related peptide (CGRP), and the sympathetic neuropeptide Y (NPY) were used. Immunohistochemistry was performed by the avidin-biotin-peroxidase method. In the innervated teeth, a correlation between increased sensory nerve density and influx of immunocompetent cells was found. Compared to the contralateral innervated molars, a significant reduction in recruitment of immunocompetent cells was found in the denervated pulp tissue subjacent to the dentinal cavities. The rat molar represents a unique model to illustrate the influence of sensory nerves and neuropeptides on inflammation and recruitment of immunocompetent cells.
牙髓是一种缺乏侧支神经供应的外周终末器官。在切断下牙槽神经（IAN）轴突后，大鼠磨牙牙髓在至少6天的时间内会失去神经支配。因此，大鼠磨牙牙髓被用作实验模型，以研究感觉神经纤维在牙本质损伤后对免疫活性细胞流入的影响。在本研究中，我们对去神经支配和有神经支配的下颌第一磨牙牙本质龋下方的CD43 +、CD4 +、CD11b +和表达I-A抗原的细胞进行了定量分析。为了可视化神经纤维，使用了针对蛋白基因产物（PGP）9.5、感觉神经肽P物质（SP）和降钙素基因相关肽（CGRP）以及交感神经肽Y（NPY）的抗体。免疫组织化学采用抗生物素蛋白-生物素-过氧化物酶法进行。在有神经支配的牙齿中，发现感觉神经密度增加与免疫活性细胞流入之间存在相关性。与对侧有神经支配的磨牙相比，在牙本质龋下方的去神经支配牙髓组织中，免疫活性细胞的募集显著减少。大鼠磨牙是一个独特的模型，可用于说明感觉神经和神经肽对炎症以及免疫活性细胞募集的影响。
