Chandrachud L M, Grindlay G J, McGarvie G M, O'Neil B W, Wagner E R, Jarrett W F, Campo M S
Beatson Institute for Cancer Research, CRC Beatson Laboratories, Glasgow, United Kingdom.
Virology. 1995 Aug 1;211(1):204-8. doi: 10.1006/viro.1995.1392.
We have previously shown that cattle vaccinated with L2, the minor structural protein of bovine papillomavirus-4 (BPV-4), do not develop alimentary papillomas upon challenge with BPV-4. Analysis of the B and T cell response in L2-vaccinated animals showed that the majority of the response was directed against the N-terminus and C-terminus of L2 with little response against the middle portion. Cattle were vaccinated with the N-terminus or the C-terminus of L2. The animals vaccinated with the N-terminus were completely protected from viral challenge, whereas the animals vaccinated with the C-terminus were not. Further analysis with synthetic overlapping peptides spanning the entire N-terminus mapped a B cell immunodominant epitope at amino acid 101-120. This epitope was recognised by all vaccinated animals.
我们之前已经表明，用牛乳头瘤病毒4型（BPV-4）的次要结构蛋白L2接种疫苗的牛，在受到BPV-4攻击后不会发生消化道乳头瘤。对接种L2疫苗的动物的B细胞和T细胞反应分析表明，大多数反应针对L2的N端和C端，而对中间部分的反应很少。用L2的N端或C端给牛接种疫苗。用N端接种疫苗的动物完全受到保护，免受病毒攻击，而用C端接种疫苗的动物则没有。用跨越整个N端的合成重叠肽进行的进一步分析在氨基酸101-120处定位了一个B细胞免疫显性表位。所有接种疫苗的动物都能识别这个表位。
