Babas T, Belhadj-Jrad B, Le Grand R, Dormont D, Montagnier L, Bahraoui E
Laboratoire d'Immunovirologie des Lentivirus des Primates, UFR SVT, Université de Toulouse III, France.
Virology. 1995 Aug 1;211(1):339-44. doi: 10.1006/viro.1995.1414.
Three mouse monoclonal antibodies (mAb) were produced against soluble recombinant vaccinia virus gp140 from SIV-mac251. Two mAbs (1B9 and 6C11) were mapped at the aa 411-430 sequence within the V4 domain, and the third mAb (3C8) recognizes a conformation-dependent epitope on the external envelope glycoprotein. This was shown by its loss of reactivity in Western blot and ELISA with dithiothreitol-reduced gp140. mAb 3C8, but not 1B9 and 6C11, cross-reacts well with gp140 and gp125 from HIV-2ROD, indicating that this discontinuous epitope includes conserved regions localized within the external envelope glycoprotein. Analysis of the neutralizing activities of the mAbs showed that only mAb 1B9 is able to inhibit both syncytium formation and SIVmac251 infection of human peripheral blood lymphocytes.
制备了三种针对来自SIV-mac251的可溶性重组痘苗病毒gp140的小鼠单克隆抗体（mAb）。两种单克隆抗体（1B9和6C11）定位在V4结构域内的第411 - 430位氨基酸序列处，第三种单克隆抗体（3C8）识别外膜糖蛋白上的一个构象依赖性表位。这通过其在蛋白质印迹法和酶联免疫吸附测定中与二硫苏糖醇还原的gp140反应性丧失得以证明。单克隆抗体3C8，而非1B9和6C11，能与来自HIV-2ROD的gp140和gp125发生良好的交叉反应，表明这个不连续表位包含位于外膜糖蛋白内的保守区域。对这些单克隆抗体中和活性的分析表明，只有单克隆抗体1B9能够抑制人外周血淋巴细胞的合胞体形成和SIVmac251感染。
