Benichou S, Legrand R, Nakagawa N, Faure T, Traincard F, Vogt G, Dormont D, Tiollais P, Kieny M P, Madaule P
Unité de Recombinaison et Expression Génétique, Institut Pasteur, Paris, France.
AIDS Res Hum Retroviruses. 1992 Jun;8(6):1165-70. doi: 10.1089/aid.1992.8.1165.
Two murine monoclonal antibodies (MAbs), designated MATG2014 and MATG2033, were generated. They are reactive with the external envelope glycoprotein gp130 of the simian immunodeficiency virus of macaque monkey (SIVmac251), and display a cell-free virus neutralizing activity in vitro. In addition, MATG2014 cross-reacts with HIV-2Rod gp140. Epitope mapping of these MAbs was performed by screening and SIVmac peptide library expressed in yeast and confirmed using synthetic peptides. MATG2014 and MATG2033 recognize two overlapping epitopes localized in an 18 residue domain between amino acid 171 and 188 of the SIVmac251 gp130. Sera from experimentally SIV-infected macaques are immunoreactive with this neutralizing domain. Sequence comparison with related SIV and HIV-2 viral strains indicates a low variability of this region, consistent with the cross-reactivity of MATG2014 with HIV-2Rod gp140. This domain should then be considered in designing experimental vaccines.
产生了两种鼠单克隆抗体（MAb），命名为MATG2014和MATG2033。它们与猕猴猿免疫缺陷病毒（SIVmac251）的外膜糖蛋白gp130反应，并在体外显示出无细胞病毒中和活性。此外，MATG2014与HIV-2Rod gp140发生交叉反应。通过筛选在酵母中表达的SIVmac肽库对这些单克隆抗体进行表位作图，并使用合成肽进行确认。MATG2014和MATG2033识别位于SIVmac251 gp130氨基酸171至188之间18个残基结构域中的两个重叠表位。实验性感染SIV的猕猴的血清与该中和结构域发生免疫反应。与相关SIV和HIV-2病毒株的序列比较表明该区域变异性低，这与MATG2014与HIV-2Rod gp140的交叉反应一致。因此，在设计实验性疫苗时应考虑该结构域。
