el Yazidi I, Boilly-Marer Y
Laboratoire de Chimie Biologique, Université des Sciences et Technologies de Lille, Villeneuve D'Ascq, France.
Anticancer Res. 1995 May-Jun;15(3):783-90.
Normal, as well as the majority of malignant, mammary epithelial cells will proliferate upon stimulation by FGFs. However estrogen-independent MDA-MB-231 cells (which are able to grow in vitro in serum-free medium) are not significantly stimulated by exogenous FGFs even though they possess high and low affinity receptors for these growth factors. Biological assays, measuring CCL39 fibroblast proliferation or PC12 pheochromocytoma cell neurite outgrowth, demonstrated the presence of FGF activity in MDA-MB-231 cell extracts and also in the culture medium conditioned by these cells. This biological activity decreased in the presence of neutralizing anti-FGF antibodies. Using immunohistochemical methods FGF1 and FGF2 immunoreactivity was detected in MDA-MB-231 cells. After purification by heparin-Sepharose chromatography and Western blot analysis, M(r) 18000 molecules showed the same physicochemical characteristics as FGFI and FGF2. These results demonstrate the production and release of FGF related molecules by MDA-MB-231 cells and suggest an autocrine stimulation of these cells.
正常乳腺上皮细胞以及大多数恶性乳腺上皮细胞在成纤维细胞生长因子（FGFs）的刺激下都会增殖。然而，雌激素非依赖性的MDA-MB-231细胞（能够在无血清培养基中体外生长）即使拥有这些生长因子的高亲和力和低亲和力受体，也不会受到外源性FGFs的显著刺激。测量CCL39成纤维细胞增殖或PC12嗜铬细胞瘤细胞神经突生长的生物学试验表明，MDA-MB-231细胞提取物以及这些细胞条件培养基中存在FGF活性。在存在中和性抗FGF抗体的情况下，这种生物学活性会降低。使用免疫组织化学方法在MDA-MB-231细胞中检测到了FGF1和FGF2免疫反应性。经肝素-琼脂糖层析纯化和蛋白质印迹分析后，18000分子量的分子显示出与FGF1和FGF2相同的物理化学特征。这些结果证明了MDA-MB-231细胞产生并释放了FGF相关分子，并提示这些细胞存在自分泌刺激。
