Renaud F, El Yazidi I, Boilly-Marer Y, Courtois Y, Laurent M
Laboratoire INSERM U450, Paris, France.
Biochem Biophys Res Commun. 1996 Feb 27;219(3):679-85. doi: 10.1006/bbrc.1996.0294.
In normal (NMEC), transformed (HBL-100) and malignant human mammary epithelial cells (MCF 7, BT-20, MDA-MB 231), we have examined the expression and the regulation by serum of FGF1 and FGF2 mRNA. FGF2 mRNA level was higher in NMEC and in a HBL-100 than in malignant cell lines (MDA-MB-231, BT-20). No FGF2 mRNA was detected in the malignant cell line, MCF-7. In contrast, the FGF1 mRNA was detected in all the mammary epithelial cells but at different levels. NMEC, HBL-100 and MDA-MB231 cells expressed similar level of FGF1 and higher than that observed in BT-20 and MCF-7. In contrast to FGF2 which is only expressed in nonmalignant cells, no correlation between FGF1 mRNA expression and the phenotype of the cells was observed. We followed the expression of four FGF1 mRNA, heterogenous in their 5' untranslated regions. This study demonstrated that (i) the FGF1 mRNA 1.A was not expressed by mammary epithelial cells, (ii) the FGF1 mRNA 1.B was only expressed in normal mammary epithelial cells and (iii) the transcripts 1.C and 1.D were expressed in normal and malignant cells with specific patterns. The expression of FGF1 mRNAs responded in a cell specific manner to serum starvation. The mRNA 1.A was only expressed in normal cells cultured in the absence of serum while 1.C was either up- or down-regulated by serum in transformed cells and the expression of 1.D was greater in presence of serum in all cell lines. These results show that the regulation of FGF1 mRNAs expression is cell specific and does not correlate with a tumorigenic or transformed cell phenotype.
在正常（NMEC）、转化（HBL - 100）和恶性人乳腺上皮细胞（MCF 7、BT - 20、MDA - MB 231）中，我们检测了FGF1和FGF2 mRNA的表达及其受血清的调控情况。FGF2 mRNA水平在NMEC和HBL - 100中高于恶性细胞系（MDA - MB - 231、BT - 20）。在恶性细胞系MCF - 7中未检测到FGF2 mRNA。相反，在所有乳腺上皮细胞中均检测到FGF1 mRNA，但水平不同。NMEC、HBL - 100和MDA - MB231细胞表达的FGF1水平相似，且高于BT - 20和MCF - 7中的水平。与仅在非恶性细胞中表达的FGF2不同，未观察到FGF1 mRNA表达与细胞表型之间的相关性。我们追踪了四种FGF1 mRNA的表达情况，它们在5'非翻译区存在异质性。这项研究表明：（i）乳腺上皮细胞不表达FGF1 mRNA 1.A；（ii）FGF1 mRNA 1.B仅在正常乳腺上皮细胞中表达；（iii）转录本1.C和1.D在正常和恶性细胞中以特定模式表达。FGF1 mRNA的表达以细胞特异性方式对血清饥饿作出反应。mRNA 1.A仅在无血清培养的正常细胞中表达，而在转化细胞中，1.C受血清上调或下调，且在所有细胞系中，1.D在有血清时表达量更高。这些结果表明，FGF1 mRNA表达的调控具有细胞特异性，与致瘤或转化细胞表型无关。
