差异显示技术鉴定茄子（Solanum melongena L.）体细胞胚胎发生过程中受发育调控的基因。

Differential display identifies developmentally regulated genes during somatic embryogenesis in eggplant (Solanum melongena L.).

作者信息

Momiyama T, Afele J C, Saito T, Kayano T, Tabei Y, Takaiwa F, Takayanagi K, Nishimura S

机构信息

Department of Cell Biology, National Institute of Agrobiological Resources, Tsukuba, Japan.

出版信息

Biochem Biophys Res Commun. 1995 Aug 15;213(2):376-82. doi: 10.1006/bbrc.1995.2142.

DOI:10.1006/bbrc.1995.2142

PMID:7544119

Abstract

Changes in gene expression patterns during early somatic embryogenesis of eggplant (Solanum melongena L.) were characterized by means of differential display. Eight products of polymerase chain reaction (PCR) derived from newly expressed mRNAs after four days of culture were identified and cloned. One clone, pTM002, was found to contain a 416 bp insert and identical to the proximal arbitrary primer at both ends. Based on the terminal sequences, reverse-transcription PCR (RT-PCR) was carried out to monitor the expression level of pTM002. The results indicated that the message of pTM002 was increased to correlate with the intensities obtained in the differential display. The significance of this method in investigation of differentially expressed genes is also discussed.

摘要

利用差异显示技术对茄子（Solanum melongena L.）早期体细胞胚胎发生过程中的基因表达模式变化进行了表征。鉴定并克隆了培养四天后新表达的mRNA衍生的八个聚合酶链反应（PCR）产物。发现一个克隆pTM002含有一个416 bp的插入片段，两端与近端任意引物相同。根据末端序列，进行了逆转录PCR（RT-PCR）以监测pTM002的表达水平。结果表明，pTM002的信息增加，与差异显示中获得的强度相关。还讨论了该方法在差异表达基因研究中的意义。