小鼠Rab geranylgeranyl转移酶β亚基的克隆、染色体定位及胚胎表达研究。

Studies of cloning, chromosomal mapping, and embryonic expression of the mouse Rab geranylgeranyl transferase beta subunit.

作者信息

Wei L N, Lee C H, Chinpaisal C, Copeland N G, Gilbert D J, Jenkins N A, Hsu Y C

机构信息

Department of Pharmacology, University of Minnesota, Minneapolis 55455, USA.

出版信息

Cell Growth Differ. 1995 May;6(5):607-14.

PMID:7544156

Abstract

The mouse Rab geranylgeranyl transferase beta subunit has been cloned from a mouse E8.5 embryonic cDNA library. Sequence comparison reveals 97.4% sequence identity at the amino acid level to the rat clone isolated from an adult rat brain cDNA library. This gene, given a gene symbol of Rabggtb, is mapped in the distal region of mouse chromosome 3. It is ubiquitously expressed in adult animals but displays an interesting pattern of expression during a specific time of embryonic development. The expression of this gene can be detected in the whole embryos during early embryonic stages and is specifically concentrated in the developing brain, heart, and liver between gestation stages of E11.5 and E13.5. In addition, the expression of this gene is induced by retinoic acid in a mouse embryonal carcinoma cell line, P19.

摘要

小鼠Rab geranylgeranyl转移酶β亚基已从E8.5期小鼠胚胎cDNA文库中克隆出来。序列比较显示，其氨基酸水平与从成年大鼠脑cDNA文库中分离出的大鼠克隆序列一致性达97.4%。该基因的基因符号为Rabggtb，定位于小鼠3号染色体的远端区域。它在成年动物中普遍表达，但在胚胎发育的特定时期呈现出有趣的表达模式。在胚胎早期阶段，整个胚胎中均可检测到该基因的表达，且在E11.5至E13.5妊娠阶段，其表达特异性集中于发育中的脑、心脏和肝脏。此外，在小鼠胚胎癌细胞系P19中，视黄酸可诱导该基因的表达。