Oulad-Abdelghani M, Chazaud C, Bouillet P, Mattei M G, Dollé P, Chambon P
Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP, Collège de France, Illkirch, C.U. de Strasbourg.
Int J Dev Biol. 1998 Jan;42(1):23-32.
We report the structure, chromosomal localization and expression features of Stra3, a novel mouse gene whose expression is upregulated by retinoic acid in P19 embryonal carcinoma cells. The Stra3 cDNA sequence, which encodes a novel member of the TGF-beta superfamily, corresponds to, but extends more 3' than the recently published lefty sequence (Meno et al., 1996, Nature 381:151-155). The Stra3/lefty protein, which exhibits characteristics of secreted proteins, is synthesized as a precursor of 42 kDa and secreted after cleavage, suggesting that it may function as an intercellular signaling molecule. There are four exons in the Stra3/lefty gene and its 5' flanking region contains, among other putative regulatory elements, an unusual retinoid response element consisting of two half sites arranged as a palindrome, with an 8 base pairs spacer. We also show that Stra3/lefty is ectopically induced in the endodermal and ectodermal layers following in vivo administration of retinoic acid to gastrulating mouse embryos.
我们报道了Stra3的结构、染色体定位及表达特征，Stra3是一种新的小鼠基因，其在P19胚胎癌细胞中受视黄酸上调表达。Stra3 cDNA序列编码转化生长因子-β超家族的一个新成员，与最近发表的lefty序列（Meno等人，1996年，《自然》381:151 - 155）相对应，但在3'端延伸得更多。Stra3/lefty蛋白具有分泌蛋白的特征，以42 kDa的前体形式合成，裂解后分泌，这表明它可能作为一种细胞间信号分子发挥作用。Stra3/lefty基因有四个外显子，其5'侧翼区除其他假定的调控元件外，还包含一个不寻常的类视黄醇反应元件，该元件由两个半位点组成，呈回文排列，中间有一个8个碱基对的间隔区。我们还表明，在给原肠胚形成期的小鼠胚胎体内施用视黄酸后，Stra3/lefty在内胚层和外胚层中被异位诱导。
