Lokeshwar B L, Lokeshwar V B, Block N L
Department of Urology, University of Miami, Florida, USA.
Anticancer Res. 1995 Jul-Aug;15(4):1191-8.
High level expression of the cell surface adhesion molecule CD44 standard form and its splice variants, have been causally linked to tumor metastasis. In this study, we investigated the significance of CD44 expression in human prostatic carcinoma cells. Immunocytochemistry showed high level expression of CD44 in cells from a high grade prostate tumor, and two androgen-independent, invasive prostatic carcinoma lines, PC-3 and TSU-Pr1. Normal prostatic epithelial cells and LNCaP, a low metastatic, androgen sensitive cell line, expressed none to a very low level of CD44, although mRNA transcripts were detected in all cell lines. Immunoprecipitation detected two proteins of M(r) approximately 140 kDa and 210 kDa in PC-3, and predominantly the M(r) approximately 95 kDa protein in TSU Pr1, but none in LNCaP. Most importantly, a neutralizing antibody to CD44 inhibited cell proliferation and basement membrane invasive activity, suggesting a definitive role of CD44 in prostate tumor growth and metastasis.
细胞表面粘附分子CD44标准型及其剪接变体的高表达与肿瘤转移存在因果关系。在本研究中，我们调查了CD44表达在人前列腺癌细胞中的意义。免疫细胞化学显示，在来自高级别前列腺肿瘤的细胞以及两种雄激素非依赖性、侵袭性前列腺癌细胞系PC-3和TSU-Pr1中，CD44呈高表达。正常前列腺上皮细胞和低转移、雄激素敏感的细胞系LNCaP表达的CD44为无至极低水平，尽管在所有细胞系中均检测到了mRNA转录本。免疫沉淀在PC-3中检测到两种分子量约为140 kDa和210 kDa的蛋白质，在TSU Pr1中主要检测到分子量约为95 kDa的蛋白质，但在LNCaP中未检测到。最重要的是，一种针对CD44的中和抗体抑制了细胞增殖和基底膜侵袭活性，表明CD44在前列腺肿瘤生长和转移中具有明确作用。
